#21

He also apparently said this in a response to Ken Wasserman:

“My corporation LXR-VT corp. Is now forming a strategic partnership with Greg Fahy (who has already demonstrated that he can rejuvenate the thymus glands of older humans), and we will soon bring rejuvenation to market the most cost-effective and ethical human rejuvenation.”

5 Likes
#22

Good finding LDV. In addition to this, Harold Katcher answered to Liz Parrish comment on linkedin “By 2050, the number of people aged over 60 is expected to more than double to 2.1 billion. So what does this mean for us financially – and for the wider economy?”.

He said:

Hi Liz, what I don’t understand is that we (I, Harold Katcher, former CSO of Yuvan Research Corp) have already clearly demonstrated that we have reversed aging and increased lifespan, and few seem to notice, not even you. I (We) have already established that aging can be reversed at the molecular, cellular, tissue, organ, organ system and organismic levels in peer-reviewed publications and with Steve Horvath being a co-author as well as Gordon Luac, yet no one seems to notice or believe our incontestable results. Perhaps because I am not from Harvard or Standford or MIT, though these schools have never demonstrated significant success in this area. After leaving Yuvan Research, I have now started a new corporation (LXR-VT Corp) which is forming a strategic alliance with famous researcher who has unambiguously demonstrated the rejuvenation of at least one important organ (I haven’t asked his permission to use his name, though he will be announcing the arrangement in his company’s newsletter. It is our intention to bring rejuvenation and life-extension to everyone who wants it, and we will succeed.

Surprisingly, I have been unable to find anything else about said LXR-VT Corp. in the net. Hope any of you have a better chance to shed some light on this company.

Just my two cents.

3 Likes
#23

Hmmm….Maybe we can get a test for E5 from Theranos? Would be nice to hear something that moves this topic away from scam and towards reality.

2 Likes
#24

Theranos. I believed in her because I wanted to. I wanted to walk into a pharmacy and get my vitamin D and ApoB done for $25. 10 minutes later. Brilliant idea.

Very important to evaluate things without bias.

#25

Katcher and Exosomes pop up yet again in a recent clip compilation from Modern Healthspan that aired 28 minutes ago:

2 Likes
#26

In case you are interested: Dr. Katcher is 80 yrs old.

"Harold KatcherHarold Katcher
• 3rd+ • 3rd+
Professor at University of MarylandProfessor at University of Maryland
2mo • 2 months ago
Follow

Dear Ezekiel,
At 80 years of age, I am living perhaps the happiest period of my life, the most creative and productive. My goal (achieved so far in rats, and my own skin) is rejuvenation. Even though my balance is shaky and walking a mile is difficult, I delight in life every day. If (when) I succeed in reversing aging, will your desire to die at 75 still remain? At age 72 I went to India to begin a new line of research on reversing age, after years of trying, I succeeded. Had I only lived to 75, the world would have been without hope. What if everyone could be “exceptional” like your colleague? I believe we could make it so (based on experimental evidence). What if all the learning and experience we have gained during a long life were not just thrown away by our deaths, but could still be employed to heal the world? I know this is possible, in fact probable and in years, not decades. Yes, physicists and mathematicians generally make their greatest contributions when they are young, but those engaged in other fields where complexity requires years to obtain (like medicine and biology, history and psychology) often make their major contributions at older ages. And yet, we have shown (with rats, so far) that even mental acuity can be restored (as well as physical abilities and health). So don’t give up on life. Human lifespan has been determined by evolutionary considerations, but our intelligence allowed us to overcome predators and famines, which is why our lives are longer than other mammals our size, but we have gone past evolutionary determinism; while it took Nature millions of years to teach reptiles to fly, and whales to live in the ocean, it took only a few thousand years for man to learn to fly (further and faster than any bird) to live beneath the ocean (longer than any whale) and to explore other planets (which no other living things have done). In a similar manner we can change our lifespans, living centuries or even millennia, thus opening up the vastness of the cosmos to us. Man, immortal in the Heavens has long been the dream of mankind. We wait for God to do it, but God works through man, His wonders to perform. So, don’t give up, a new world is just beginning.
Harold’

5 Likes
#27

Latest update on Katcher’s E5.

Sounds like he had some “partner” issues but has regained control of his baby!

Future Plans and Challenges

Dr Katcher recently parted ways with Yuvan Research due to limitations on his autonomy, but he remains committed to advancing E5. With a well-equipped laboratory in Salt Lake City, Utah, and a team of technical experts, he is focused on making E5 commercially viable for widespread use. Despite having some investments, Dr Katcher is open to additional funding to accelerate research and development.

3 Likes
#28

I think the problem is that it requires a lot of work refining piglet blood to create any of it (and thats before we know whether it is much use for human beings anyway).

I think the mechanism is a form of mitochondrial transplant through EVs.

4 Likes
#29

Finally a new paper (Open Access) on the E5 experiments:

E5 treatment showing improved health-span and lifespan in old Sprague Dawley rats

Aging and, in particular, the emergence of age-related disorders is associated with tissue dysfunction and macromolecular damage, some of which can be attributable to accumulated oxidative damage. In the current study, we determine the potential of ‘plasma-derived fraction (E5)’ for cellular rejuvenation and extending the lifespan of Sprague Dawley (SD) rats. This is a unique study wherein we have used 24-month-old rats and monitored them until the end of their lifespan with and without E5 treatment. In the present investigation, the SD rats were separated into two groups old control group and the treatment group (n = 8). The treatment group received four injections of E5 every alternate day for 8 days, and eight injections every alternate day for 16 days. Body weight, grip strength, cytokines, and biochemical markers were measured for more than 400 days of the study. Clinical observation, necropsy, and histology were performed. The E5 treatment exhibited great potential by showing significantly improved grip strength, remarkably decreased pro-inflammatory markers of chronic inflammation and oxidative stress, as well as biomarkers for vital organs (BUN, SGPT, SGOT, and triglycerides), and increased anti-oxidant levels. Clinical examinations, necropsies, and histopathology revealed that the animals treated with the E5 had normal cellular structure and architecture. In conclusion, this unique ‘plasma-derived exosome’ treatment (E5) alone is adequate to improve the health-span and extend the lifespan of the old SD rats significantly.

https://onlinelibrary.wiley.com/doi/full/10.1111/acel.14335

7 Likes
#30

Authors and Affiliations
Department of Human Genetics, David Geffen School of Medicine, University of California, Los Angeles, CA, USA

Steve Horvath

https://www.biorxiv.org/content/10.1101/2023.08.06.552148v1

Horvath remarked, “Initially, I could hardly believe the profound epigenetic rejuvenation effects of E5. However, our findings are robustly supported by parallel rodent studies from different labs.” Upon final analysis by Horvath and his team, there was a remarkable 67.4% average reversal in the epigenetic age of treated elderly rats. If these results translate similarly to humans, it could equate to an 80-year-old reverting to the age of 26.

Exosomes sourced from another species reverses biological age of rats by more than 50%

3 Likes
#31

Sima, the rat that lived for 44 months is mentioned as the star of the rats in the study and Akshay Sanghavi, the CEO of Harold Katcher’s former company, is a co-author, so this isn’t a new study. Guess we’ll have to wait a while longer to find out what’s been happening since his association with Greg Fahy.

1 Like
#32

So, other than the paper noted by @RapAdmin, this is all old news right?

#33

Yes, I think everything on E5 is in Limbo right now - the company founders split up, Harold Katcher is trying to make a go of it himself, but he’s probably not fundable from any typical investment source (he’s almost 80 years old).

It seems like this science should go open source - and be tested by people here. Perhaps someone can contact him and get him to share the in-depth details on the forum of his technique, and people can begin to crowd source implementation details, and figure out a way to try it safely in humans.

4 Likes
#34

I’d rather see it verified by Miller in the ITP, but I guess he won’t do injections. It’s not been repeated in rodents in this country has it? Or dogs? It’s not within even years of humans.

But I still love the open source idea and actually Harold acts like he wants the world to have this and that it should be within reach of everybody. So that part isn’t crazy. I thought maybe Fahy could make the money machine go brrrrr.

1 Like
#35

I don’t think it has - but if it became open source then biology college students and others using biohacking labs could create their own small-scale studies and we could get a lot more information a lot faster. These local Biohacker spaces are always available:

the local LA bio maker spaces / or general “Maker” spaces:

https://lamakerspace.org

USA-WEST

Berkeley BioLabs – Berkeley, CA

Bio, Tech and Beyond – Carlsbad, CA

Denver Biolabs – Denver, CO

La Jolla Library Bio Lab – La Jolla, CA

Biodidact – Los Alamos, NM

TheLab – Los Angeles, CA

Counter Culture Labs – Oakland, CA

PortLab – Portland, OR

DIYbio San Diego – San Diego, CA

Wet Lab – San Diego, CA

Indie Bio – San Francisco, CA

HiveBio Community Lab – Seattle, WA

BioCurious – Sunnyvale, CA

Source:

1 Like
#36

One thing that could prevent biohackers from replicating E5 and using it is the amount needed. Dr. Katcher said it’s four times the volume of the old exosomes - for each infusion, I think. He also said that U.S. pigs aren’t a good source because of the antibiotics they’re given - with Indian pigs there wasn’t that problem.

From what little I’ve heard about bioreactors, possibly they could solve the volume issue - if not now, then when they become more commercially viable.

Several people have mentioned that E5 infusions could be paired with plasma dilution to get rid of the old exosomes, making the young exosomes more effective. Couldn’t that reduce the volume requirement?

Also, he doesn’t seem to consider E5 a finished product. Effective in its current state, yes, but maybe better with some refinement.

3 Likes
#37

Josh Mittledorf posted a narrative on his blog setting out his theory of aging. Toward the end, he briefly mentions three studies rejuvenating old rats with young exosomes, done in Mumbai, somewhere in China and in Los Angeles. The one in Mumbai uses exosomes from young pigs, obviously Harold Katcher’s study, but the other two use exosomes from young rats.

I was unaware of the Los Angeles and China experiments. Does anyone here know anything about them?

1 Like
#38

I think he’s discussing this study here:
https://www.nature.com/articles/s43587-024-00612-4

#39

Dr. Mittledorf may have meant this study, but he did say the other two were rat studies. Dr. Katcher used rats because the blood vessels of mice were too small to infuse plasma reliably. Apparently these researchers were able to overcome that problem.

Another possible difference is their reference to extracellular vesicles instead of the subcategory of exosomes - their filtering may have resulted in exosomes, or exosomes plus other factors, or other factors without exosomes. They described how they did it, but it’s beyond me.

At first glance, it seems like they’re doing about the same thing as in the rat study - no reason to assume it’s not as important - the more replications there are, the sooner the concept could go mainstream. Thanks for the link.

1 Like
#40

I know he said that, but I’ve seen studies where they’ve done I.V. on mice before. I don’t remember the specifics.

exosome therapy can vary dramatically based on concentration. In the Chinese study, they mentioned this:
To simulate the natural concentration of sEVs in plasma, the purified sEVs were dissolved in PBS and adjusted to a concentration of 1.80 μg of total protein per microliter.

However, I believe Katcher’s E5, it was concentrated far more than the ‘natural concentration’. I could be wrong here… but I remember this being mentioned.