Thanks. It’s a full time job to have any chance to do it right. I already have a full time job. Until I figure it out, I am simplifying to keep the risks low.
Thoughts so far (a work in progress):
- a limit on number of non food chemicals I include in my active stack. 5-20? I can rotate between stack A & B, etc.
- a limit on how long I will take non food chemicals without a break. Rapa gets a 1 month holiday every 3 months
- a limit on what I will take with rapamycin. Mostly I will stop everything else on rapa day -1, 0, +1
- full blood tests every 3 months. React swiftly to negative changes (pullback)
- strong reaction (pullback) to negative changes in functional metrics: RHR, sleep, joint / muscle soreness, brain power, mood, BP, metabolic flexibility (sensitivity to sugar, hunger during fasting)
- low AGEs, 30 plants per week, cycled protein volume, no alcohol, limited eating window, while food…diet.
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@Olafurpall Could you share your medication/supplement stack with the rest of us? You seem like a highly intelligent and well researched individual. I’d like to compare and discuss if you wouldn’t mind? 
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@Joseph_Lavelle Great strategy. I especially like the strong pullback when there is a negative response such as muscle soreness. I should have pulled back faster with the statin.
So far there are only 3 things that have given me a bad physical reaction - Rosuvastatin (intense muscle pain), beta-alanine (whole body itchiness) and magnesium citrate (joint pain + diarrhea).
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AnUser
#24
I have thought about this more, and this is probably why I’ll probably skip this entire Lustgarten approach. I’ve tried tracking all of my food and it’s a bit of a hassle. I haven’t tried taking a lot of blood tests every year but now that I think about it I don’t want to do that. Instead I’ll focus on the easy approach to healthspan/longevity, like longevity drugs, using statins (later on PCSK9i when I prioritize paying for it), rapamycin, etc and be more of the “hedonistic wing” of biohacking as @John_Hemming puts it and focusing on wellbeing instead with of course some easy (taking pills) longevity interventions, for example. I only want to do blood tests every now and then to check for apoB etc, and I guess I can put some other things to do at the same time like CRP and basic function of different organs.
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Taking a blood test a week as i do mainly (maybe 48 tests) a year is quite expensive.
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AnUser
#26
At the moment I feel like I don’t want I want the entire blood drawn process multiple times a year, once every couple years maybe, unless I think it has a lot of value. Do you alternate between arms like Lustgarten? Since I live in a previously socialist country I think the nurses will roll their eyes if I do it as well. The attitude here I think is that only people who are sick need to get their blood drawn as determined by a doctor, even if I choose and pay for my own blood tests.
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As a principle i aim for the same arm. Normally it has completely repaired during the week.
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I thought i would provide a photo. I last had blood drawn on wednesday. I think one reason injuries repair more slowly in older people is a shortage of cytosolic acetyl-coa. As people know i supplement to increase acetyl-coa levels.
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AnUser
#29
I think it might depend on the nurse’s skill or luck as well. One time my arm was a mess at the blood drawn spot and it kind of hurt a little bit IIRC, was pretty dark.
That’s true. There are some dreadful phlebotomists. However, I have had blood taken from this spot on these dates (as well as earlier ones): 13/07/2023 19/07/2023 28/07/2023 03/08/2023 10/08/2023 16/08/2023 06/09/2023 12/09/2023 12/09/2023 21/09/2023 27/09/2023
If people wish to experiment with speeding up repair to injury I am happy to talk about that. It is the same citrate protocol as I use for improving differentiation.
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What exactly is your supplementation protocol in this area?
There are two routes towards increasing acetyl-CoA (outside the mitochondria). One involves increasing citrate and the ACLY enzyme them converts that to acetyl-CoA. The other involves increasing acetate and the ACCS2 enzyme converts that to acetyl-CoA. The latter has an element of self-inhibition which is a nuisance.
You can increase citrate in a number of ways. In my view the best thing to do is to eat it, but there are complications that I go into on this thread:
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Another point people may find interesting is that you can increase acetate by either drinking vinegar (I don’t think that is good myself because of the acid) or in fact by drinking alcohol and waiting for the liver to turn the acetaldehyde into acetate. You actually end up with a reasonably high concentration of serum acetate.
However, I think citrate is a better target. It is not necessarily as much fun, however.
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JuanDaw
#34
The researchers used data gathered for the Singapore Chinese Health Study, in which 63,257 Chinese people aged 45-74 were followed from 1993 to 2010.
When there were only low amounts of ALA in their blood, EPA and DHA reduced the chance of a heart attack by 18 percent, according to the figure above. At a high ALA concentration, the reduction was as high as 49 percent.
Source publication.
https://www.sciencedirect.com/science/article/pii/S0022316622089969?via%3Dihub
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I am tracking the repair process from venepuncture over the first day and subsequent days for the blood test I did at 1pm today. Would you like me to post results?
stealle
#36
Did you ever directly answer this question?
This thread answers the question.
tfl.phd
#38
I would also add, that it is quite common for omega-3 supplements to be rancid (over 45% of omega 3 supplements).
That’s of course, not considering that many omega-3 supplements are also not IFOS-certified for toxins and overall purity.
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Paper on omega-3 consumption decreasing epigenetic age as gauged by second-generation epigenetic clocks.
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L_H
#40
John, does apple cider vinegar increase acetyl coa? And do you know the dose response curve given the self-inhibition?
