#82

It would be interesting to see any papers on this. I accept that my dosing of p5p is less than pyridoxine and that may be why I am not getting negative effects which I did get from 100mg a day of pyridoxine.

#83

This is basic textbook knowledge. As an example, here is a quote from Advanced Nutrition And Human Metabolism, Fifth Edition:

“For vitamin B6 to be absorbed, the phosphorylated vitamers must be dephosphorylated. Alkaline phosphatase, a zinc-dependent enzyme found at the intestinal brush border, or other intestinal phosphatases hydrolyze the phosphate to yield either pyridoxine (PN), pyridoxal (PL), or yridoxamine (PM).”

#84

I did a cursory search and found

and

But they are old papers and may have been superseded.

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#85

How much P5P do you take?

#86

Yes Perhaps just a coincidence, but will stop.

#87

If it’s 1 pill daily then I would think it’s unlikely that your neuropathy was caused by the B6, but there is still a small chance of that so it’s a good idea to take some months off to see if it makes a difference. Good luck.

#88

Its something in the 30s.

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#89

According to @ConquerAging in his latest video, the two blood biomarkers most predictive of longevity are RDW and Lymphocytes.

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#90

How does he calculate this?

Interestingly my high dose experiment increased the proportion of lymphocytes temporarily.

#91

I think everyone can(assuming no major physical activity), but it depends on where those calories are coming from. 40% less calories it means around 1500 calories per day. I buy organic steaks at my local grocery shop and is listed at 600cal (12 OZ) and if you eat one lunch, and one dinner with broccoli it comes to about 1500-1600. that is 1.5lbs of lean organic meat, and I know for sure it is plenty for me (I’m a big guy btw). But if I eat two muffins same calories, I’ll be hungry all day long.

Obviously, I’m not saying one can only eat steaks and broccoli for rest of their life, but chicken breast and other meats and lean fish are also very low in calories yet satiating. Plus, you can have 10lbs of vegies and barely make 400 Calories lol. But if most of calories are coming from fat, pasta, dairy and bread etc., then you’ll most likely be hungry 24/7 on 15-1600 calories per day.
I think the calorie intake suggestions are NOT accurate, certainly not the same for everyone. I once counted calories for about 5-6 weeks and never had more than 1800/day but mixed foods, and did NOT lose one single Lb. My supposed daily calories intake should be 2500, and by lowering it to 1800 I was supposed to lose about one Lb per week, lost none, zero, zilch. to be fair I wasn’t exercising or being physically active at that time but still I was very disappointed at the time (over 20 years ago).

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#92

When restricting calories you are supposed to weigh every single item of food you ingest or beverage you drink and use specific apps like cronometer. Otherwise, the total can be very misleading.

Also, -40% calories for a human being is usually too much. CR in humans is most aptly calculated as a % of weight lost, taking as a reference your weight in early manhood.

Let’s remind that Bryan Johnson has been slowly decreasing his % of CR with time.

#93

What’s the typical impact of rapamycin on RDW and lymphocytes (if any)?

Anecdotal evidence in this thread suggests that rapa might lower RDW and lymphocytes: Any Rapamycin users have high Lymphocytes?

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#94

There was an MIT study that showed Rapamycin lowered RDW in vitro.

Also the link you provided showed members lymphocyte values went up after taking Rapamycin. So that’s good.

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#95

Given that RDW is the range of Red Blood Cell sizes and this can vary during the life time of a red blood cell (about 120 days) it is not a good metric when people use intermittent rapamycin as rapamycin will reduce RBC size and therefore increase the variety of sizes.

The levine formula uses the percentage of lymphocytes and sees a lower WBC count as being good.

Again this is not really a good metric when people are using rapamycin as can be seen from my WBC results.

WBC went down from 2.7 to under 1.9 and back up again.

The lymphocytes percentage went up then back down

29.03% 40.69% 36.19% 35.75% 35.45% 38.04% 41.03% 36.61% 25.93%

I have edited my other table to include the lymphocytes percentage.

As a matter of curiosity I have used the levine spreadsheet to see what the implication of that variation would be in years. Lymphocyte percentage is a 1.98 year variation and WBC is 0.49 years.

Ie when the effect of Rapamycin was at its peak on each of those biomarkers my levine age would have been 2.47 years younger.

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#96

In rereading this interesting thread containing so many good ideas, it seems pretty clear for some metrics and likely for many others that a subsuming/subsumed relationship exists such that knowing metric x obviates the benefit of and therefore the need to know metric y. In statistical terms, thinking of all of these metrics as independent variables regressed against mortality or lesser dependent variables, it seems likely that a relatively small number of metrics will account for most of the variance in the main dependent variable. If so, the cost (direct and opportunity) of measuring and managing subsumed metrics is not especially justifiable. I’m speaking generally at this point but I believe the sparse but robust set of metrics I am hypothesizing could be derived using current data. If so, it would be a beneficial undertaking for the NIH or similar entity.

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#97

Its hard to do this. Morgan levines algorithm is a good try, but has flaws. For example reall high albumin is a bad sign, but the algorithm thinks it is good.

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#98

What I’m proposing is empirical. Individually, there would be no metrics that did not account for the greatest obtainable proportion of the variance, although there could be interactions running in different directions that would be fruitful to explore…

#99

It is kind of easy actually. You weight vegies once in one of the containers that I use (then look at cal serving chart of the package), plus I know cals for steak, plus cals for two soft boiled eggs and one table spoon of olive oil as an example. When I’m measuring calories I don’t eat 20 different foods. Usually, stick with 3-4 common foods that I already know the calories for each portion.

#100

I tend to look for functional tests, blood biomarkers are a good example of this. However, they are not easy to handle as data points as their natural variation is high and then there can be testing issues.

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#101

That works after you have been weighing every single item for weeks.
Fatty foods govern in CR. Small mistakes can result in wrong assumptions on CR.
The above being said, if you are sure, I would like to have your metabolism and be able to keep my weight on 1800 kCAls.
But on the other side being physically active sure burns a lot, sometimes more than expected.

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