#41

Please read this. Anyone interested must understand that a very specific protocol must be followed to avoid negative consequences.

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#42

Long discussion that includes some details on HBOT mechanisms re: hyperoxia-hypoxia paradox here Oxygen, hypoxia and hyperoxia

IIRC the consensus in that thread was that HIF was likely not induced with typical (m)HBOT protocols and the benefits must be due to some other mechanism. Eg hyperoxia/hyperpenetration of low oxygen/injured tissues. HIF seems to have a fairly narrow window of activation

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#43

If that were the consensus, it is not a consensus I agree with.

I think the positive effects from HBOT relate mainly to the stimulation of HIF 1 α

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#44

Fair. Here is one of the posts I was referring to.

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#45

I looked at a lot of papers a few years ago and wrote this

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#46

I haven’t looked into whether or how much of the benefits of HBOT therapy would be explained by hypoxia, but we know from a lot of studies that do not use oxygen breaks that hypoxia is unlikely to be necessary for majority of the benefits. I guess people interested in aging benefits and looking at the Efrati studies are just wanting oxygen breaks to copy his protocol exactly, just in case the oxygen breaks are required for the benefits.

#47

There is obviously an oxygen break at the end of the hyperbaric session.

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#48

Good point. That would be one long break at the end of the session compared to 3 short breaks and one long one during a 90 minute session using the Efrati protocol. So four times fewer breaks. is that enough to get close to the hypoxic benefits of the Efrati protocol? I don’t know.

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#49

Olafurpall wrote
“The claims of HBOT increasing telomere lenghts are questionable at best”

Is your conclusion caused by the lack of a control group and the limited group size (n=35)?

From

Results: Telomeres length of T helper, T cytotoxic, natural killer and B cells increased significantly by over 20% following HBOT. The most significant change was noticed in B cells which increased at the 30th session, 60th session and post HBOT by 25.68%±40.42 (p=0.007), 29.39%±23.39 (p=0.0001) and 37.63%±52.73 (p=0.007), respectively. There was a significant decrease in the number of senescent T helpers by -37.30%±33.04 post-HBOT (P<0.0001). T-cytotoxic senescent cell percentages decreased significantly by -10.96%±12.59 (p=0.0004) post-HBOT. In conclusion, the study indicates that HBOT may induce significant senolytic effects including significantly increasing telomere length and clearance of senescent cells in the aging population

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#50

For me the results are crystal clear.

#51

I will receive tomorrow, after 3 months since order from China directly from manufacturer, a 2.0 ATA with mask.

I plan to use it according to Dr Shari Efrate protocol alternating hyperoxia (15min) to normoxia (5min), in order to achieve maximal effect related to HIF-1a (but also telomere, mitochondria volume, ATP capacity by increasing vo2max, brain performance, etc).

I’ve measured telomere length both via truediagnostic and via dantelabs (with the latter we are developing together a comprehensive transcriptome differential analysis for longevity, but still WIP), and expect to enlong it and maintain over life at a certain length.

Still have to buy a battery emergency power supply, to reduce electrical risks at home of a pressure drop, but at least tomorrow will receive it and can starts playing with it.

Paid $30k including transport and custom clearing to Italy via Rotterdam, with a tight negotiation with supplier, my wife is Chinese, we checked that seems safe enough being used in Chinese hospital (and many European brand, are just OEM rebrand of Chinese products, the only European “producers” are Turkish).

I am keeping going in increasing the toolkit, while looking forward retire for work, to have some more serious time and commitment to hack by body by 2026.

Attached the protocol from Dr. Efrate book plus the tech sheet of the chamber I took.

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#52

Hey!

Thanks for sharing this!

Do you think I could get the same price with delivery to Germany?

#53

I am also thinking of getting one of these but would need to convince someone it won’t be too onerous. Two issues that might be an issue are noise and power consumption. Do you know how I’d answer these - i.e. be able to say its silent and consumes about the amount of boiling a kettle three times (for example :slight_smile: )

#54

I think will costs likely the same, can make introduction to them within the pre-negotiated pricing, but I’d advice to let me test and try it first :wink:

3 Likes
#55

I’ve been at hbot center (soft chambers only), and in general the oxygen concentrator components are a bit noisy tough.
Soon as i “unpack” and plug the new toy will make a video to share the noisy level.

Fabio

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#56

Does the chamber have normal oxygen levels? I presume so because of the mask, that should be a priority and measured in some way without increasing fire risk. (I’d use o3-pro or something to research this further + reading of my own papers, but being vary of hallucinations from the models).

I wouldn’t wear any clothing or bring anything to the chamber either because of the fire tail risk, is this recommended? Maybe some way of reducing static electricity before entering? What else :thinking:

I would want to have real time monitoring of oxygen levels were correct in the chamber and provided by the mask unless it increases risk in other way that doesn’t make it worth it or someone is monitoring me.

(Just some of my reasoning traces).

1 Like
#57

No. While those are certainly problems, my conclusion is more based on the fact that even if the study was large and well controlled their results still wouldn’t prove that telomere lengths actually increased in the people.

Efrati did not exactly find that HBOT increases telomere length in humans. The results of his study can be interpreted in many ways and that it increased the telomere lenth of the cells is only one of the possible interpretations. The increased telomere length might as well be caused by movement of immune cell populations to and from the blood, or by increased leukocyte turnover. This last could be harmful because increase turnover could accelerate turnover of hematopoietic stem cells and speed up telomere shortening and senescence of those cells. As an example, one study found that HBOT accelerated the proliferative activity of epidermal cells in vitro. PMID: 29855082 We know that increased proliferation can lead to short term benefits (such as for wound healing) but also long-term harm by inducing replicative senescence

Efrati did not exactly find that HBOT increases telomere length in humans. The results of his study can be interpreted in many ways and that it increased the telomere lenth of the cells is only one of the possible interpretations. There is no way to know whether the HBOT therapy actually increased telomere length of some cells, or whether it just changed the fluxes of different types of cells to and from the blood. I hope it increases telomere length but it’s too early to claim that IMO. If the measured telomere length in the blood cells was still increased months after HBOT therapy was finished, then we could be more confident that it might actually increase telomere length.

These results are interesting but to realize why they don’t prove that telomere length increased, think of this analogy. If you make some drastic changes in New York which result in the average biological age of people there decreasing by 5 years over a 10 years follow-up, that doesn’t mean the people there got younger as in rejuvenated. Sure, it’s possible they got younger, but maybe the changes just resulted in more old people dying, or more young people moving to New York and old people moving away, or more people having children there. In that case the average age change reflects changes in the population there not the aging of the individuals there. HBOT is a bit of an extreme therapy that could very well change the population of immune cells in the blood and influence immune cell proliferation and movement to and from different compartments in people. So the longer telomere length in T-cells might have been caused by increased T-cell proliferation or death of older T-cells or something other than the telomere of the T-cells lengthening. Telomere length of immune cells in the blood is unfortunately a poor marker of telomere length. I would be more convinced that HBOT increased telomere length if they would have tested it maybe 6 months after finishing HBOT therapy (at which point any short term effects on immune cell fluxes would have been gone) and still found longer telomere length.

I hope HBOT increases telomere length and it’s possible, but the evidence is far from convincing IMO and the hype is not warranted.

3 Likes
#58

Ok please try it and then give me feedback in some weeks. Many thanks!

#59

All right, yes, before placing myself in a 2.0 ATA chamber I’d need to become an expert of hbot safety procedures, including the electrical safety, the clothing to avoid any static charges, independent oxygen level measurement.

I do think it would take still some months before engaging in using it as part of anti-aging protocol.

I’d love also to evaluate a full body MRI and microbiome analysis to make a differential analysis before and after, like i saw when i visited avivclinics.com in dubai (the clinics of dr. Efrate), to evaluate vascularization overall.

I will share the experience and place an updated blog post on hackingbiology.com and here.

Fabio

2 Likes
#60

Why don’t you start a YouTube channel?