#1

In mice …

Nice experiment

Serum analysis revealed that four months of CR treatment in mice (CR serum) activated AMPK in mouse embryonic fibroblasts (MEFs), primary hepatocytes, human embryonic kidney 293T (HEK293T) cells, and primary myocytes. This activation was determined by estimating the phosphorylation levels of AMPKα (pAMPKα) and substrate acetyl coenzyme A carboxylases (pACC).

Perfusing CR serum into mice on an ad libitum diet led to the activation of AMPK in the liver and muscle. Experimental findings also indicated the presence of heat-stable and low-molecular-weight metabolites in CR serum that could activate AMPK.

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#2

That’s a new way of making money, do a lot of CR & let people buy your serum.

[I think I’m joking … ]

#3
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#4

Probably wouldn’t take it orally:

Lithocholic acid is a secondary bile acid formed from primary bile acids through bacterial modification in the intestines. It is the most toxic bile acid and can lead to cholestasis, a condition characterized by decreased bile flow and accumulation of bile components in the liver and blood.

But a fecal transplant from mice that are caloricly restricted helps? Didn’t say how long that would last.

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#5

LCA has been reported to have anti-aging effects in yeast,[10][11][12] and more recently fruit flies, nematodes, and mice.[13] [14]

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#6

See also the discussion there: Bile acids like lithocholic acid and conjugated taurine bile acids? How to increase?

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#7

see recent eric topol substack proteomics, it is correlated with pro-aging effects (in studies that are prolly not fully controlled0