Josh
#312
"Dear MMC Sponsor,
I wanted to reach out personally and give you an update on some of the ongoing research at Ora Biomedical that includes one or more of your sponsored interventions. You may have already reached out, but in case you hadn’t we wanted to get in touch to let you know that your outstanding data is still on its way to you (and the MMC leaderboard if you opted-in to open-access). I know this is outside our expected turnaround time and I very much want to thank you for your patience.
As the sponsorship program launched, then quickly grew, we found ourselves in need of additional research infrastructure and processes to expedite retesting of interventions flagged during our QC. Over the last three months, we have scaled our team up, built tracking processes, and overcame a reagent issue that caused many interventions to require retesting. Producing the best quality, most reliable data as quickly as possible has always been our mission. We have fallen short of that with one or more of your tests. I greatly apologize and want to assure you that these issues are in the past.
Your remaining intervention tests are being conducted now at no additional cost to you. Many of these tests are close to concluding and we will expedite the return of sponsored data as soon as the experiments wrap-up. We sincerely thank you for your continued support of Ora Biomedical and the Million Molecule Challenge."
Im confused about test at no cost since we already paid. Maybe they mean retested no cost (with fix)?
4 Likes
Bicep
#313
It says “no additional cost” and they had a reagent issue that required retesting.
Am I correct here in saying that so far I don’t think of the results from this have been helpful in terms of identifying geroprotective molecules? I love the idea, especially the crowd sourcing, relative simplicity, and the open nature of the results.
However:
Rapamycin has failed every dose tested
The ones which do work seem to be ‘gaming’ the assay itself, such as antibiotics which affect the food source. I highly doubt they’re working on fundamental longevity pathways.
Worms don’t have heart attacks, kidney issues or glucose/insulin issues. Thus things like Metformin, SGLT2i etc also fail. Fair enough. Those are more like treating age-related diseases IMO.
Berberine is really puzzling. Probably the most interesting on the leader board IMO.
Therefore, so far it seems we haven’t identified anything which prevents aging on a more fundamental basis. The search continues.
3 Likes
Josh
#315
It was always the Million Molecule challenge not the 100. And rapamycin does work in conjunction with other things as do other mtor inhibitors like the one all over the leaderboard.
Really just the one leading antibiotic that is suspect, but other studies show that it provides some life extension even with uv killed food.
3 Likes
Josh
#316
It looks like there’s been an update with various smaller doses winning against standard, and we even see Rapamycin on leaderboard now above a lot of the GSK mtor combos
4 Likes
adssx
#317
3 results:
Ursodiol (UDCA) +5% with long-lived controls (poke @Yoo):
Methylcobalamin -10%:
Methotrexate -10%:
UDCA failed in the ITP. I’m waiting for the TUDCA results.
3 Likes
Interesting that methotrexate failed. I wonder how comparable the immune system is of worms to mice to humans. Was the dose used a chemo or autoimmune level of dosing?
Notably, the leaderboard does not have any other immunosuppressive/modulating drugs, aside from Rapamycin/other mTOR inbibitors.
Curious
#319
There have been some problems with the wormbot. I got a weird result.
Order-9191-Doxycycline-HCl-50uM-±-GSK2126458-10uM-report_final.pdf (416.5 KB)
1 Like
Curious
#322
I will write to them now…And if my worms is still alive then it is world record 
3 Likes
adssx
#323
Longevity escape velocity!
1 Like
Curious
#324
Yes I have reached longevity escape velocity for worms. Xprize, the money is mine…
5 Likes
Curious
#325
Great. They answered almost immediately, and they say that the results in my both experiments are all good. They terminated the worms that are still alive in the experiment after 30 days. So I think I can celebrate a life extension success with my two experiments. I have a few ideas.
"Hi !
The data is QC’d and accurate 
Both of those treatments had very impressive lifespan extension results!
As a little context, we typically only run experiments for 30 days. In both of those experiments, by the time we ended the experiment (Day 31 for Order 9191 and Day 36 for Order 9168), there were still a large proportion of animals still alive! For 9191, we were unable to resolve a median lifespan by the time we ended the assay due to this impressive lifespan extension!
Doxycycline is a known lifespan extending intervention in C. elegans. In our system, antibiotics typically extend because we use live (as opposed to killed) bacteria as a food source. It is very interesting to think about whether an mTOR inhibitor like GSK2126458 would extend in the context of doxycycline. To better understand that we would need to run each compound separately in addition to the combination. Would also be interesting to evaluate the combination in the context of killed food. Let us know if you are interested in any further tests and I’ll send over a custom study quote.
Best wishes and thank you again for contributing to the Million Molecule Challenge!
Dr. Mitchell Lee (He/Him)
CEO & Co-Founder
Ora Biomedical, Inc."
Ora Results.pdf (501.2 KB)
Ora Results 2.pdf (261.9 KB)
10 Likes
cl-user
#326
Really impressive indeed!
They mention that " Doxycycline is a known lifespan extending intervention in C. elegans. In our system, antibiotics typically extend because we use live (as opposed to killed) bacteria as a food source."
Do we know by how much?
If they have more food, that’s the opposite of CR but then mTOR inhibition might compensate.
Eat more proteins, take Rapamycin!
2 Likes
Curious
#327
I asked for a quotation about the cost for repeating the exepriments with killed food.
5 Likes
Just remember that worms are not the best model to use for mammal longevity. What works for worms may not work for mammals and vice versa. It provides a good starting point for mammal trials though. I think if you tried doxycycline in the ITP, you wouldn’t get the same results.
2 Likes
Curious
#329
I got the quotation from Ora Medical. To repeat the previous successful experiments, but now with killed food, which costs extra $50 to do, so 250 + 250 = $500
Doxycycline HCl 50 µM + GSK2126458 10 µM
Lithium citrate 25 µM + GSK2126458 25 µM
And at the same time test the following substances also using killed food: Doxycycline HCl 50 µM, GSK2126458 10 µM, Lithium citrate 25 µM and Azithromycin also at a dose of 25 µM. This will be $600 .
Including tax etc., the total sum will be $1,583.72. At this stage this means I will not go through with these experiments will killed food. But If there are others who will join me in a crowdfunding effort, then I will pay $400.
anyone here that is interested?
2 Likes
Josh
#330
Ive been sponsoring through vida útil.io with no tax and even a small tax deduction for me. Im happy to help anyone who has to pay a tax. I do want to sponsor gsk with killed food. Is @adssx doing any killed food testing yet?
1 Like
adssx
#331
What’s the point of killed food? (I didn’t follow the whole conversation)
