#543

No, 10 mg/day of SS-31 for ten days, followed by ten days of MOTS-c at 10 mg/day.

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#544

MOTS-c has an ~4hr elimination life and is fully metabolized in a few hours. Regardless of dose. Yes a high dose will have a high peak and more area under the curve but the end point of full metabolization remains the same.

Since our body produces MOTS-c in response to physical “stress” and the amount of MOTS-c we produce declines as we age, I decided to add MOTS-c to my daily routine at a low dose of 250mcg per day.

I’ve had people say “what about de-sensitization, you need to cycle” and my response is… My body has been making this peptide (and 7,000 more) since before I was born and never became desensitized… What my body has done, is reduce the output of many things, including peptides like MOTS-c so my approach is to try and return certain peptide levels to what they were when I was younger… and never got desensitized

I believe influencers use that word to appear more “intelligent” :slight_smile: I think, that for many peptides that are bio-identical or a fragment, it’s irrelevant. For anabolics, SARMs and a few non-human peptides it does apply.

Just my 0.02 from reading a lot of studies. Here is one overview that references a few studies

> Studies have shown that blood MOTS-c levels in young people are 11% and 21% higher than those in middle- and old-aged people, respectively

Another article with references

MOTS-c in relationship to a popular longevity drug Metformin

MOTS-c - Diabetes, and Aging-Related Diseases
https://e-dmj.org/journal/view.php?number=2725

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#545

Do you happen to know any international reputable (someone has tested them for efficacy) source for peptides?

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#546

Steve, how do you dose the Mots-c?

#547

After reading the paper on diabetes, I think I will change my MOTS-c protocol from twice yearly to EOD for a month, which aligns with Dr. Seeds’ recommendations. He also suggests that MOTS-c can be cycled with other mitochondrial peptides, such as SS-31. So my protocol for MOTS-C would be 250 mcg EOD for a month, followed by 250 mcg of SS-31 EOD for a month. On the the other days, I will continue to squeeze in my TB-500/BPC-57/GHK-cu combination, also at 250 mcg of each.

The choice of 250 mcg is based more on economic than medical considerations. As you said elsewhere in this thread, I don’t have unlimited wealth.

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#548

Review posting;

And

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#549

I added it to my morning BPC+TB dose. Which I take daily

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#550

“Initial dose of 2mg the day someone gets sick or if I feel off, then 1mg/day thereafter. 10mg total. I think I’ve now done this 4 times this year and it has worked every time”

Assuming you mean thymosin alpha 1: Have you seen anything about reconstituted TA1 only supposed to be kept for a maximum of two days refrigerated?

#551

The 2-7 days timeframe was spread far and wide by the NIH article that quoted a single vendor’s statement on their product page (mybiosource dot com). The same site says it is only stable lyophilized for 3 weeks, and that has been disproven multiple times by independent testing. It’s stable for at least months before reconstitution. Some will use sterile phosphate buffered saline if they’re worried reconstituted shelf life, though.

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#552

Is there a general dilution ratio to reconstitute let say 10mg of any peptide, or is it specific to individual peptides? and what happens if the dilution is less or more (i.e. does it affect the effectiveness or the maximum days it can be kept)? BTW, I’m not interested in how dosing is calculated since that is literally math 101 lol. thanks.

#553

Dilution doesn’t affect efficacy, so it’s really a matter of practicality. A desired dose of 1mg is easy (dilute with 1 ml so that the draw for each dose is 10 units). If your desired dose is 100 ug, then ideally you’ll want to dilute a lot more so that you aren’t trying to draw a 1 unit dose on an insulin syringe, which is next to impossible to do with any real accuracy.

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#554

Ok, so basically, I can dilute any 10mg peptide (most well-known ones) with one 1ml for example and it will be fine? The reason why I’m still keeping the question open is because a week or so ago I read somewhere a chart of dilution for most peptides and some of them had different ratio suggestions, for example let say BPC157 was 10mg to 1ml while CHK CU had it say 10mg to 2ml (unfortunately can’t find that chart now, but I know for a fact that there were different ratio recommendations for some). That’s why I was wondering if there is a specific reason other than what @Davin8r suggested is for practical reasons only.

Thanks for dosing explanation but that part I think is the easiest to figure out and believe it or not it’s the most explained all over the web, and I’m like who the hell do these people think they are dealing with :joy:. I thought all people in western world finish at least elementary school lol, but I understand how it can initially be a bit confusing especially when you dose in mcg or ug units.

Anyway, if anyone has anything specific about the dilution, please chime in but please keep away from how to come up with dosing portions. Thanks,

#555

Sleepdoc, do you mind describing some of the effects?

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#556

@Davin8r has the right answer. I usually don’t do less than 2mL per vial (but sometimes 1, sometimes 3) to get dosing accuracy and to avoid wastage when trying to get the last few drops out. Though you can always inject a little more solution in at the end.

Here’s Tailor Made’s peptide catalog. Scroll to the dosing charts section in the back showing all the different concentrations they use. It confirms what @Davin8r said.

For semaglutide and tirzepatide, you can also look at the pharma concentrations.

25835966 (1).pdf (2.4 MB)

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#557

Thanks @Davin8r and @AgentSmith. My question has been answered.
WOW the catalog is exactly what I (didn’t know) was looking for. Thanks again @AgentSmith

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#558

Hey Steve,
I’ve had chrondomaclia in my knees for almost 40 years. I would like to do more impact to assist my osteoporosis, but I have limits on what I can do pain free. Would one of these things help something that is not acute/new?

I do perfectly just fine day to day, so I have no interest is doing anything that could cause harm, but thought I’d throw this out there?

#559

I tried both - found Semax very effective and Adamax not at all. Just on my second test of Semax with similar results to the first time. Unfortunately, whilst my brain seems to be functioning like that of a 20 year old, my muscles aren’t responding in a similar fashion and I’ve managed to get fairly painful inflamed rotator cuffs with over exercising. I’m taking it easier this time around.

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#560

I have not seem much in the studies I’ve read with respect to osteo and chondromalacia and BPC + TB. Understanding the root cause and addressing that would be the first thing to consider and that is way beyond my education level :slight_smile:

They are not “pain releivers” per se as the pain relief comes from the injury being healed.

BPC has a benefit for soft tissue, ligaments and tendons. TB-500 similar benefits through a different pathway.

Here is some info on peptides that may help in is area.

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#561

Some peptides affect the pH of the final solution and “burn” when injected. Two solutions;

  1. use more diluent so there is less effect on the pH of the dose
  2. use Phosphate Buffered Saline solution

Some peptides “gell” if there is not enough diluent, one solution

  1. use more diluent
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#562

That’s a false statement
A minimal dilution is necessary to lower the risk of protein aggregates that lead to an immunogenicity effect, wich can be problematic long term. FDA require lab pharmaceuticals to study this phenomenon on their injectable protein-based drugs. Every change in concentration has to be studied again before approval

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