AnUser
#4
Just combine with Orchid genome embryo screening which you should do either way.
59vw
#5
Certainly possible but that approach requires artificial insemination. Eggs are fertilized in a petri dish, embryos are screened by removing a cell and sequencing, and then some are selected for implantation. This of course involves a couple more bars for a successful pregnancy in an otherwise marginal situation. One could of course screen pregnancies and abort those with aneuploid genomes. I guess I was just pointing out that although this bodes well for Rapa it might not be the panacea that it initially appears to be.
2 Likes
Ilya_M
#6
Any ideas where this numbers came from? They mean humans, don’t they?
Found it. Apparently they mean mice after all.
1 Like
Yes - it must have been the early (lower dose) ITP trials they are talking about… I don’t know why they quote the earlier ones and not the higher 23% to 26% seen in later ITP trials.
1 Like
adssx
#8
You wrote “a much lower dose”, but they used 5mg/week which is the average that people use here, no?
We don’t split out male and female dosing, but yes, I suspect thats probably about average (or perhaps a little higher) for the females here taking rapamycin. If you looked at our poll, he’s probably right (a significant percentage are higher than the 5mg per week), but those are mostly probably heavier males… What is the Rapamycin Dose / Dosage for Anti-Aging or Longevity?
2 Likes
adssx
#10
Good point on weight. We need the full paper to have the average weight of participants but being American 40yo females we can expect about 77 kg (vs 90 kg for the average man the same age). So 0.065 mg/kg, which gives 6mg/week for a 90 kg person.
Anyway, great news that will hopefully bring more women here!
1 Like
Perhaps I’m wrong. I thought most people were taking 8mg. Given its long half life that means you’re constantly topping up every week and will reach saturation without cycling. I take only 4mg myself. Means one day a week I’m completely clear of rapa by my calcs.
EDIT: I’m very light. 67kg.
1 Like
The highest frequency dosing is 6mg/week per our survey, but we didn’t ask the sex of the responder, nor the weight, so we really don’t have enough information to draw any conclusions…
1 Like
Right so 73% are taking 6mg or more. That’s quite in line with my impression from speaking with people directly. I would assume that a large majority of people both in here and on that survey are men from what we know about online forum participation in general. Guesstimating here but I think most are not flushed of their previous weekly dose by the time they take their next one. So risk of mTOR suppression becoming constant rather than intermittent.
Average dosage according to that chart is 8.27mg.
adssx
#14
What about the paper published on rapa use? (forgot its name…) I think the average dose was 6 mg/week there, and the overall distribution was lower than in this forum’s survey.
And indeed, weight matters a lot.
[EDIT: More than the dose, I found that the most impressive was the short duration of the study: after only 3 months of rapa use they saw these massive benefits!]
Senben
#15
How did they come with number of 5 years of prolonged fertility? If they say Rapamycin could decrees ovary ageing by 20% and average menopause age is 51 and average year of starting a period is 12, than this is span of 39 yeas of periods and 20% is 7,8 years.
Also, study lasted for 3 months, how they come to conclusion that it will prolong periods for approx. 5 years? The hypothesis that Rapamycin slows down ovarian ageing by suppressing numbers of released eggs from 50 to 15 per months is based on premises that there is a final number of eggs in woman ovaries and there are new researches which suggest there are ovarian stem cells in ovaries which can produce new eggs. There are woman who wrote on this forum about taking Rapamycin after menopause and getting they periods again which would not be possible if there was a final number of eggs in ovaries. Also, Rapamycin regenerates organs which can be maybe the case in ovaries too, so delaying menopause cold be also from this aspect, not only form “preserving” numbers of eggs in ovaries.by suppressing mTOR.
1 Like
adssx
#17
Thanks.
73 women (average weight 62 kg), 260 men (average weight 81 kg). Too bad the graph is in # and not %, and they should have given the doses per kg of weight as well, but anyway, both the median and the average for men and women seem to be around 5 mg/week, which is exactly what was used in this fertility study.
1 Like
Without disagreeing with this point, I would think the problem of a reduction in mitochondrial quality is also likely to be an issue. The reason paternal age is not as important to fertility as maternal age is that the paternal mitochondria are either destroyed or irrelevant in the quantity in the zygote.
I find it interesting that the human egg cells are created in the mother well before the mother herself is born:
I see that as a solution to the problem of gradually deteriorating mitochondria (which I personally see as a primary store of biological age). Hence for babies to be born young they need to come from egg cells which were created when the mother was an embryo and kept in a relatively quiescent state since then.
eli
#19
Females in the study were ( at least according to cited source) only up to 35 years old , so probably younger than several of us here…
I also understand none of them would have had side effects of headaches etc …I just had my 5 mg dose yesterday ( taken at 11.55 am ) and was totally out as of 16 pm until mid this morning due to atrocious headache 
(it felt like I was suddenly hit hard by the flu but luckily it mostly passed by now) Not sure this was since I paused it for 3 weeks ( had some 5 mg doses before with mostly just huge fatigue and a rash once in a while) but not sure if I will up my dose further . I guess it is ok to take ibuprofen or paracetamol against the headache? ( I did take some as I could barely move from the pain yesterday)
KarlT
#20
Would mean or median be of more use here?
1 Like
Eli, was this your first dose? or have you been taking it for a while and suddenly got a headache this time? Did you start low (1mg/week) and work up, like many of use do? What is the Rapamycin Dose / Dosage for Anti-Aging or Longevity?
Median would be in fairness. At a glance it looks like it’d be lower as well.
Senben
#23
There are more research saying that DNK fragmentation in sperm can be cause for infertility in couples. DNK fragmentation sperm analysis are now used in IVF clinics, before it was only sperm quality and quantity. Also age of a men has impact on lower sperm quality and quantity which can impact fertility… As, I already mentioned ovarian stem cells are a recently discovered type of cell than can give rise to oocytes in culture and produce healthy oocytes in vivo. The work of Harvard Sincalir Lab may overturn the dogma that a female is born with a set number of eggs that are simply lost over time due to damage and genomic instability. They goal is to identify genes and small molecules that can reactivate ovarian stem cells in vivo to treat premature ovarian failure, chemotherapeutic ovarian failure (in cancer patients) and extending the healthy and fertile period for women. https://sinclair.hms.harvard.edu/research
1 Like
