Esp the pasture raised eggs?

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Microplastic Contamination in Eggs vs. Other Common Foods

Introduction

Microplastics – tiny plastic particles typically smaller than 5 mm – have become widespread contaminants in food and water. Studies have detected microplastics in many foods we consume, raising concerns about human exposure through diet. Common food categories including seafood, meats, dairy, grains, vegetables, and fruits have all been found to contain microplastics to varying degrees. This report compares microplastic contamination levels in chicken eggs with those in other major food groups, based on peer-reviewed studies and credible food safety sources. We also note whether eggs tend to have lower microplastic loads than other foods, and highlight any regional or source-related variations in contamination. A summary table is provided to compare average microplastic levels across these food types for easy reference.

Microplastics in Eggs

Recent research confirms that chicken eggs can contain microplastics, though at relatively low levels. A 2022 study in Food Chemistry optimized methods to detect microplastics in eggs and found an average of about 11.7 microplastic particles per egg. Most of these particles were 50–100 μm in size, predominantly polyethylene plastic, and they were more concentrated in the yolk than the egg white. Notably, cooking did not significantly change the microplastic count in eggs, implying these particles persist through common food preparation. Another study of eggs in Iran (Bushehr port region) reported similar low contamination – roughly 4 microplastic particles per egg on average. These studies suggest that while microplastics do make their way into eggs (likely via hens ingesting contaminated feed or water), the levels are on the order of only a few to a dozen particles per egg. This is a relatively low count compared to many other foods, as discussed below.

Microplastics in Seafood

Seafood (fish and shellfish) is often highlighted as a significant source of microplastic exposure, since marine environments are heavily contaminated with plastic debris. Indeed, numerous studies have documented microplastics in fish tissues and shellfish. For example, one estimate suggests that people who are high shellfish consumers might ingest up to ~11,000 microplastic particles per year just from shellfish like mussels and oysters. Bivalves are filter feeders and are often eaten whole, so their microplastic load can directly transfer to consumers. Fish can also contain microplastics (particularly in the gastrointestinal tract), but filleted fish muscle tends to have fewer particles.

A comprehensive 2024 study examined 16 different protein foods and found microplastics in 88% of all seafood and meat samples tested. Interestingly, this study noted no significant difference in microplastic concentrations between seafood (ocean-sourced proteins) and terrestrial meats. In other words, fish and shellfish were just as likely to contain microplastics as beef, pork, or chicken in that sample. The levels in seafood varied widely depending on the product and processing: minimally processed fish fillets had very low counts (on the order of a couple of particles per serving), whereas highly processed seafood products had much higher levels. For instance, breaded shrimp (a processed seafood product) contained on average 370 ± 580 microplastic particles per serving, whereas a raw skinless fish fillet contained only a few particles. Overall, seafood can be a notable source of microplastics – especially shellfish or processed fish products – but raw fillets may have relatively low levels (comparable to other meats).

Microplastics in Meat (Beef, Pork, Poultry)

Like seafood, terrestrial meats have been found to harbor microplastics, though generally at low levels in raw form. The same 2024 protein study mentioned above found microplastic contamination in 88% of meat samples including beef, pork, and chicken. The lowest contamination among the tested proteins was in a plain chicken breast, at only about 2 ± 2 microplastic particles per serving (~100 g portion). Similarly low counts (single-digit particles per portion) were observed in other unprocessed meats like steak or pork chops. These results indicate that raw meat muscle tissue contains very few microplastic particles – likely because most ingested plastics in livestock stay in the gut or other organs rather than the muscle we eat. However, when meats are processed or ground, contamination tends to increase, presumably due to contact with plastic equipment or packaging. For example, products like chicken nuggets or ground beef patties showed higher microplastic counts per gram than whole cuts of meat.

In addition to muscle cuts, microplastics have also been detected in animal blood and organs, as well as in animal feed. A 2022 Dutch pilot study (Free University of Amsterdam and Plastic Soup Foundation) found plastic particles in all cow blood samples tested, and in 7 of 8 beef samples (meat) analyzed. This study also found microplastics in all tested feed pellets given to cattle. The presence of plastic in feed and animal blood explains how microplastics can end up in meat and milk. Still, for a consumer eating a piece of steak or chicken, the number of microplastic particles ingested is generally very low (often just a few particles), especially if the product is unprocessed. In summary, eggs and raw meats are both on the lower end of microplastic contamination compared to many other foods, although processed meat products can have higher levels.

Microplastics in Dairy

Dairy products, particularly milk, can also contain microplastics introduced through feed or processing. The Dutch study mentioned above tested cow’s milk and found microplastics in 18 of 25 milk samples (72%) from farm and supermarket sources. Most of the detected particles in milk were at very low concentrations (often near the analytical detection limits). There was no significant difference between organic and conventional farms in terms of milk contamination. Another recent review noted that since 2020, at least 17 studies worldwide have confirmed the presence of microplastics in dairy products (including cow’s milk and even human breast milk). These findings indicate that milk and dairy can contain trace microplastic fibers or fragments, likely originating from feed, plastic milking equipment, or packaging. For instance, plastic tubing used in milking machines and plastic milk bottles or caps could shed microparticles into the milk. However, the absolute levels in liquid milk appear to be very low – on the order of a few particles per liter in many cases (with some samples showing no detectable particles above the quantification limit). By comparison, solid dairy products (cheese, etc.) are less studied, but they could similarly pick up contaminants during processing or storage. Overall, while dairy is not microplastic-free, the typical contamination levels (a few particles per sample) suggest that eggs often have comparable or even slightly higher microplastic counts (per item) than a glass of milk, though both are quite low relative to some other foods.

Microplastics in Grains and Cereals

Staple grains and their products can contain microplastics, often introduced during processing, packaging, or from contaminated irrigation water. Rice is one grain that has been closely examined. A study in 2021 found that people could be consuming 3–4 mg of microplastic for every 100 g of rice. Instant rice was even more contaminated, with around 13 mg of plastic per 100 g serving. These values in milligrams correspond to many thousands of microscopic particles (including nano- and micro-scale plastics) per serving, given how light each particle is. The source of rice contamination may include plastic debris in paddy fields or particles from processing machinery and plastic packaging. Washing rice before cooking can reportedly reduce microplastic contamination by up to ~40%, hinting that some particles adhere to the rice surface from processing and can be rinsed off.

Other grain products have also shown microplastic presence. For example, researchers have found microplastics in wheat and corn flour, dried pasta, and bread, usually at low levels (a few particles per gram or less), likely coming from milling equipment or plastic storage bags. Sea salt – while not a grain, it’s a common crystalline food seasoning – often contains microplastics as well. Surveys of commercial table salt worldwide found the majority of brands (over 90%) contained microplastics, with sea salts containing on the order of a few hundred particles per kilogram. This could translate to a handful of particles ingested via salt per person each year (one analysis estimated <12 microplastics per person annually from salt intake). Compared to grains and processed grain foods (which can have dozens or more particles per serving in some cases), eggs generally have lower microplastic loads (only single digits per egg). However, it’s worth noting that much of the microplastic in grains are extremely small (<0.5 mm and even in the micron scale), whereas the few particles found in eggs or meats tend to be slightly larger on average.

Microplastics in Vegetables

Earlier research assumed that plants might not absorb microplastics, but recent studies have overturned that notion. Vegetables grown in soil can take up micro- and nanoplastic particles through their roots, transferring them into edible tissues. The first study on microplastics in fruits and vegetables (Oliveri Conti et al., 2020) revealed surprisingly high contamination levels. Median microplastic counts in sampled vegetables were on the order of 10^5 particles per gram of tissue. Carrots were the most contaminated vegetable tested, and lettuce the least, yet even lettuce had a median of about 52,000 particles per gram. Carrots and other root vegetables can accumulate plastic particles from the soil, especially very tiny particles (in the 1–10 μm range) that can travel up the plant’s vascular system. Leafy vegetables like lettuce had fewer particles by comparison (tens of thousands per gram, as noted), possibly because of differences in root structure or growth time. These numbers are orders of magnitude higher than those found in animal products. However, it’s important to recognize that these vegetable studies counted particles down to ~1 μm in size, which vastly inflates the counts (since a single larger microplastic could break into many microscale fragments). In any case, on a particle-count basis, vegetables (and fruits) are among the most microplastic-contaminated foods reported. By contrast, an egg (≈50 g by weight) with ~10 particles total equates to ~0.2 particles per gram – hundreds of thousands of times lower than the counts reported in carrots or apples. Thus, eggs typically have far lower microplastic load than vegetables in terms of particle counts.

Microplastics in Fruits

Fruits show a similar contamination trend to vegetables, as they can also absorb micro/nanoplastics from soil and water. The same 2020 study found that fruits had even higher microplastic levels than vegetables on average. The median level in fruit samples was about 223,000 particles per gram. Among fruits tested, apples were the most contaminated, with well over 100,000 microplastic particles per gram of apple. Pears were also high on the list. These astonishing numbers are attributed to the highly developed vascular system in fruit pulp and the longer growing period of fruit trees, which allow more time and pathways for tiny particles to accumulate. Again, these counts predominantly consist of very small particles (~1–2 μm). For perspective, an average-sized apple (150 g) could contain on the order of 15–30 million microplastic particles if the concentration is truly ~10^5 per gram. Most of these are nanoplastics that are currently difficult to assess for health impact, but they do indicate widespread environmental plastic infiltration. By comparison, a chicken egg with ~10 particles total is negligible in particle count. Even if we consider the total mass of plastic, an apple’s microplastics might amount to tens of micrograms of plastic, whereas an egg’s might be a few micrograms at most. Thus, eggs have markedly lower microplastic contamination than fruits by any measure reported so far.

Regional and Source-Related Variations

Microplastic contamination in foods can vary by region and source due to differences in environmental pollution and processing practices. For example, sea salt produced in Asia tends to contain higher microplastic counts than European sea salt, reflecting greater plastic pollution in some Asian coastal waters. One analysis of commercial salts found Chinese sea salts averaging 550–680 particles per kg, whereas some European salts had medians around 466 particles per kg. Likewise, seafood from heavily polluted areas may carry more microplastics. Fish caught near urban coastlines or in plastic-polluted seas often show higher plastic loads in their guts than fish from cleaner waters.

For eggs, regional data are still limited, but the available studies hint at some differences. The Chinese market eggs study (Jiangnan University) found roughly 12 particles per egg, whereas the Iranian study (Bushehr port) found about 4–5 per egg. This could suggest regional variation in contamination (perhaps due to differences in feed, farming practices, or local environmental microplastic levels). However, it could also be due to different detection methods or particle size cut-offs between studies. There was little difference between “commercial” and “home-raised” eggs in the Iran study, implying that backyard chickens and farm chickens in the same region were similarly exposed.

Processing vs. fresh sources is another important factor. The protein foods study in the U.S. showed that highly processed foods contained more microplastics per gram than fresh or minimally processed foods. For instance, a factory-made fish stick or plant-based burger had significantly higher microplastic counts than a fresh fish fillet or a home-cooked beef steak. This points to contamination introduced during processing – e.g. plastics shed from machinery, conveyor belts, cutting boards, or packaging. Yet, interestingly, even wild-caught fish (processed minimally) had some microplastics, and the study did not find a statistical difference between processed and unprocessed categories overall when all samples were considered. This suggests multiple sources of contamination (environmental + processing) can contribute concurrently.

In summary, where and how a food is produced can influence its microplastic burden. Generally, foods from more polluted environments or those undergoing extensive industrial processing will have higher microplastic contamination. Eggs, being a closed product formed inside an animal, tend to have consistently low microplastic levels across different contexts, especially when compared to plant-based foods grown in open environments.

Comparison of Microplastic Levels Across Food Types

The following table summarizes typical microplastic contamination levels reported for eggs and various common food categories, based on recent scientific studies. (Values are approximate and intended for comparison; different studies may use different detection size thresholds and units.)

Food Category Typical Microplastic Level (Approx.)
Eggs (chicken) ~4–12 particles per egg (≈50 g)
Seafood – Fish Raw fillet: few particles per 100 g (e.g. ~2 particles in a 100 g fillet); Processed (e.g. fish sticks): tens to hundreds per serving (up to ~370 in breaded shrimp).
Seafood – Shellfish Tens of particles per portion (e.g. ~0–10 particles per individual mussel/oyster); high consumers may ingest ~11,000/year.
Meat (beef, pork) Whole cuts: ~1–5 particles per 100 g portion (often near or below detection); Processed/ground: higher but generally <50 particles per portion (varies with processing).
Poultry (chicken) Whole cuts: ~2 particles per 100 g (per chicken breast); Processed (nuggets, etc.): can be higher (a study found nuggets had more MPs/g than raw meat).
Dairy (milk) Typically 0–5 particles per ~500 mL (most milk samples had none above quantification, some had a few). Presence in ~72% of tested samples (trace levels).
Grains (rice) 3–4 mg of microplastics per 100 g rice (uncooked); ~13 mg per 100 g instant rice (equating to thousands of particles; washing can reduce ~40%).
Grains (others) Wheat, corn products: generally low particle counts (est. <1 particle per gram), mostly from processing (exact numbers vary by study). Sea salt: median ~466 particles/kg in Europe (~0.5 particle per gram).
Vegetables Tens of thousands of particles per gram on average. Carrots ~>100k/g and Lettuce ~52k/g reported (particles mostly 1–10 μm in size).
Fruits Hundreds of thousands of particles per gram. Apples ~100k–200k/g (highest among fruits), largely <5 μm particles.

Note: The units above differ – some are counts per item or per gram, others are mass of plastics. Nonetheless, a clear trend emerges: eggs have markedly lower microplastic contamination than most other foods, especially when compared on a per-gram basis. Fruits and vegetables show the highest counts (due to uptake of tiny particles), whereas animal-derived foods (eggs, milk, meat) generally contain only a few particles, mostly introduced via feed or processing. Seafood sits intermediate – a fillet is low like other meats, but certain seafood (shellfish or heavily processed fish products) can deliver higher exposures.

Conclusion

Eggs generally have lower microplastic loads than other common foods. In peer-reviewed studies, chicken eggs contained on average only a handful of microplastic particles each, which is negligible compared to the tens of thousands per gram found in some fruits and vegetables. Seafood has a reputation for microplastic contamination, and indeed shellfish can carry significant particle loads, but recent evidence shows land-based foods are not far behind. Meats and dairy tend to have minimal microplastics in raw form (comparable to eggs on a per-serving basis), though processing can increase their contamination modestly. Regional differences and production methods (wild vs. farmed, processed vs. fresh) can influence contamination levels, but the overall pattern holds: plant-derived foods (especially produce and grains) often contain far more microplastic particles than animal-derived foods like eggs, milk, or meat.

It’s worth noting that many of the particles in produce are extremely small (micron or sub-micron size) and their health significance is not yet well understood. By contrast, the few particles found in eggs or milk are larger fragments or fibers that may be easier to detect and possibly filter out or avoid. Ongoing research is needed to fully assess the human health implications of consuming microplastics. In the meantime, these findings help prioritize exposure sources – for instance, reducing plastic in agricultural practices and food processing could meaningfully cut down the microplastic content in our diet. Eggs, being relatively less contaminated, might be considered a lower-risk food in terms of microplastic exposure, especially when compared to certain fruits, veggies, or processed foods. However, as one scientist aptly noted, “There’s no way to hide from plastics if you’re eating” – virtually all food categories have some level of contamination, reflecting the pervasive presence of microplastics in our environment and food supply.

Sources:

  • Liu et al. (2022), Food Chemistry: Microplastics in eggs (China study)
  • Milne et al. (2024), Environmental Pollution: Microplastics in common proteins (USA study)
  • Oliveri Conti et al. (2020), Environmental Research: Microplastics in fruits & vegetables (Italy study)
  • Dutch Plastic Soup Foundation study (2022): Microplastics in milk, feed, beef (Netherlands)
  • Van Cauwenberghe & Janssen (2014): Microplastics from shellfish (Europe)
  • Catarino et al. (2018): Microplastics from seafood (UK)
  • Additional data compiled from EPA reports, Ocean Conservancy briefs, and news summaries referencing peer-reviewed studies.
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if raw egg has lower protein bioavailability, does it mean i absorb FEWER calories from the same mass (this is MOST important for me)

[this might be true]

Honestly I think you are correct although I don’t have any scientific sources atm to back my speculation. I read a book years ago titled Catching Fire: How Cooking Made Us Human that discussed the topic and it might have something. I will skim through the book and see what I can find.

Short answer

Yes. Because roughly half of the protein in a raw egg escapes digestion, you net ≈ 9-10 kcal fewer per large egg than if you ate the same egg fully cooked. The fat calories are absorbed almost completely either way, so the calorie gap is driven almost entirely by the loss of digestible protein.


Where the numbers come from

Component in one large egg (≈ 50 g) Raw – % actually absorbed Cooked – % actually absorbed Metabolizable kcal (raw) Metabolizable kcal (cooked)
Protein ≈ 6 g (4 kcal / g) ≈ 51 % of amino-N absorbed ≈ 91 % absorbed 6 g × 0.513 × 4 = 12.3 kcal 6 g × 0.909 × 4 = 21.8 kcal
Fat ≈ 5 g (9 kcal / g) ~95 – 100 % absorbed† ~95 – 100 % absorbed 45 kcal 45 kcal
Trace carbs < 1 kcal < 1 kcal
TOTAL ≈ 58 kcal ≈ 68 kcal

† In vitro work shows virtually complete lipolysis for hard-boiled/poached eggs, and only a few-percentage-point decline (or even a slight increase) for raw yolk depending on the digestion model .

Calorie difference: about 9–10 kcal per egg (≈ 14 %) .

Put differently, you would have to replace ten cooked eggs with raw ones just to shave ~100 kcal from your day.


Why the protein “disappears”

  • Protein structure. Heat unfolds ovalbumin, ovotransferrin, etc., exposing cleavage sites for pepsin and pancreatic proteases.
  • Trypsin inhibitors & avidin. Cooking partially inactivates these factors, which otherwise slow proteolysis and trap biotin.
  • Ileal loss. The undigested peptide–N in raw-egg meals shows up in ileal effluent (human ileostomy model) or as lower ¹³CO₂ recovery in breath, confirming true malabsorption rather than simply slower absorption .

Some of that nitrogen is later fermented by the colonic microbiota into short-chain fatty acids, so the net energy loss is slightly smaller than the 9–10 kcal headline—but still real.


Practical implications for a calorie-conscious eater

Question Take-home
Is it a big enough difference to matter for weight control? Not really. The caloric “saving” is modest and easy to erase with a teaspoon of oil.
Is eating raw eggs a safe strategy? Raw shell eggs carry a non-zero Salmonella risk (≈ 1 in 20 000 eggs) and prolonged raw-egg use can induce marginal biotin deficiency. Pasteurised liquid eggs are safer if you insist on raw texture.
Better low-calorie options? • Use egg whites (≈ 17 kcal each, almost pure protein) or cholesterol-free egg substitutes.• Keep the yolk but poach/boil instead of frying in fat.• Simply eat fewer eggs; the nutrient density of cooked eggs is otherwise excellent.
Does cooking method affect calories? Yes—mostly through added fat or water loss, not through changes in intrinsic macros. Scrambling in 5 g of oil adds ~45 kcal—swamping the 10 kcal you “saved” by going raw.

Bottom line

  • Raw vs cooked: ~10 kcal less absorbed per large egg when eaten raw.
  • Magnitude: Small; unlikely to be a meaningful weight-loss lever.
  • Safety: Raw eggs pose infection and micronutrient-absorption risks—calorie control is better pursued with portion size, leaner preparations, or separating whites and yolks.

If you still prefer raw eggs (e.g., in smoothies), choose pasteurised products and balance the marginal calorie drop against the safety trade-offs.

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Oxysterol Levels in Eggs: Scrambled vs Other Preparations

Background: Cholesterol Oxidation in Eggs

When cholesterol in foods is exposed to oxygen and heat, it can form oxysterols (cholesterol oxidation products, COPs) such as 7-ketocholesterol (7-KC). These compounds have raised health concerns due to their cytotoxic and pro-atherogenic effects . Eggs and egg-derived products are among the main dietary sources of oxysterols , especially when eggs are processed or cooked at high temperatures. The extent of cholesterol oxidation in eggs depends on cooking method and storage conditions. Below we summarize published data on oxysterol (particularly 7-ketocholesterol) content in scrambled eggs made from fresh whole eggs, and compare it to other cooking methods (hard-boiled, fried, poached) and to powdered egg products. We note whether data come from direct chemical analyses of foods or from human dietary studies.

Oxysterols in Scrambled Eggs (Fresh Whole Eggs)

Scrambled eggs made from fresh whole eggs are typically pan-cooked, exposing the beaten yolk and white to heat and air. Direct analytical data specific to “scrambled” eggs are limited, but they should be similar to other pan-fried egg preparations. In lab-based chemical analyses, fresh raw egg yolk contains essentially no detectable 7-ketocholesterol . Once cooked in a pan, however, some cholesterol oxidation occurs. For example, one study found fried egg yolk (yolk from a pan-fried egg) contained about 9.5 µg of 7-ketocholesterol per gram of yolk . In contrast, the raw yolk had none, indicating that the 7-KC was generated during cooking . Scrambled eggs (which involve similar pan frying of the yolk) would be expected to have a comparable 7-KC level on the order of 5–10 µg/g in the yolk. When considering the entire scrambled egg (yolk + white), the 7-KC concentration is diluted by the egg white mass (egg whites contain no cholesterol). By rough estimate, a two-egg serving of scrambled eggs (≈100 g) might contain on the order of 0.2–0.3 mg of 7-ketocholesterol in total. (For comparison, two large eggs contain ~370 mg cholesterol, so only a tiny fraction is oxidized under typical scrambling conditions.) These values are derived from chemical analysis of cooked eggs .

Notably, a 2013 food chemistry study on omega-3-enriched eggs also measured total COP levels in different cooking methods. Although it did not specifically test “scrambling,” it compared pan-frying vs. boiling. That study found pan-fried eggs contained about 13.6 µg of total COP per gram of egg, whereas boiled eggs had about 10.1 µg/g . (Raw eggs were ~9 µg/g in that analysis .) This suggests that scrambling/frying increases oxysterol formation slightly compared to very gentle cooking, but the rise is modest (on the order of a few micrograms per gram). The data come from a laboratory chemical analysis (J. Agric. Food Chem. 2013) .

In summary, fresh scrambled eggs likely contain only a few micrograms of 7-ketocholesterol per gram, i.e. a few tenths of a milligram per typical serving, when cooked under normal conditions. This is a relatively low level – for most people, scrambling an egg introduces only minimal oxysterols. (For context, one analysis concluded a normal mixed diet provides only minor amounts of COPs .) Still, any heat exposure does produce some oxysterols, so scrambled eggs have more than raw eggs, but far less than extensively processed egg products.

Comparison with Other Cooking Methods (Hard-Boiled, Fried, Poached)

Hard-boiled eggs (cooked in-shell in water) generally show very low cholesterol oxidation. The boiling water (100 °C) is a relatively moderate temperature and the yolk is not directly exposed to air during cooking. In fresh hard-boiled eggs, 7-ketocholesterol is often undetectable or only trace levels – effectively negligible in chemical analyses . For example, fresh egg yolk had no measurable 7-KC before cooking, and even after boiling we would expect only minimal formation (many studies don’t even report a value, implying it stays near detection limits). In the omega-3 egg study mentioned, the boiled eggs’ total COP (~10 µg/g) was only slightly above raw levels , consistent with minimal oxidation. Thus, poached eggs (cooked gently in simmering water out of the shell) likewise should have minimal oxysterol formation, since poaching is even gentler (lower temperature and shorter time) and similarly avoids direct contact with air. In fact, nutrition experts note that poaching or soft-boiling eggs helps reduce cholesterol oxidation compared to high-heat frying .

Fried eggs (e.g. sunny-side up or fully fried in a pan) involve higher surface temperatures and exposure to oxygen, so they tend to develop more oxysterols than boiling/poaching. As noted above, pan-fried eggs showed ~13–14 µg/g total COP in one analysis . Specifically for 7-ketocholesterol, fried egg yolk had about 9.5 µg/g of 7-KC . For perspective, if one fried a large egg (≈50 g total, ~17 g yolk), that equates to roughly 0.16 mg of 7-KC per egg in the yolk. This is still a small amount, but higher than in boiled eggs. The data for fried eggs come from laboratory chemical analyses of the egg yolk after frying .

Scrambled eggs vs. fried eggs: Since scrambling is essentially a form of pan-frying (with the yolk broken and mixed), the oxysterol levels in fresh scrambled eggs should be similar to those in a fried egg. Some sources hypothesize that scrambling (which incorporates air into the egg mixture) could oxidize cholesterol slightly more; however, any difference between scrambled and a typical fried egg is likely minor. In fact, one study measuring overall lipid oxidation (via TBARS) in enriched eggs found hard-boiled eggs had slightly higher oxidation than scrambled , though that was measuring peroxidation of omega-3 fats, not specific oxysterols. In terms of 7-ketocholesterol and COPs, both scrambled and fried eggs (fresh) remain low (on the order of a few μg/g), with boiled/poached lowest and frying/scrambling slightly higher.

To summarize the comparisons, Table 1 (below) compiles representative values from the literature:

Table 1. Oxysterol content (especially 7-ketocholesterol) in eggs by preparation method. Values are given roughly in µg of 7-ketocholesterol per gram of egg (or per gram of yolk where specified). For reference, 1 µg/g = 1 ppm. Data are from chemical analysis studies unless noted.

Egg Preparation 7-Ketocholesterol (µg/g) Notes & Source
Raw egg yolk (fresh) 0 (none detected) No 7-KC detectable in fresh yolk . Total oxysterols ~1.2 µg/g dry weight . (Lab analysis)
Hard-boiled egg (whole/yolk) ~0–1 µg/g (very low) Near zero 7-KC expected; boiling yields negligible COPs . (Lab analysis)
Poached egg (whole) ~0–1 µg/g (very low) Similar to boiled; gentle cooking minimizes cholesterol oxidation . (Inference)
Pan-fried egg yolk (fresh) ~9.5 µg/g 7-KC Fried in skillet, yolk portion . Total COP ~13.6 µg/g in whole egg . (Lab analysis)
Scrambled eggs (fresh whole) ~2–5 µg/g (estimated in whole egg) Yolk fully mixed/cooked – similar to fried. E.g. ~9 µg/g in yolk , which is ~3 µg/g in whole egg (0.2–0.3 mg per 100 g). (Lab analysis extrapolation)
Egg powder (spray-dried, fresh) ~1–5 µg/g 7-KC (initial) If made under mild conditions, fresh dry egg has only traces of oxysterols . One analysis found whole egg powder ≤4.6 ppm 7-KC . (Lab analysis)
Egg powder (stored) Up to 100+ µg/g 7-KC Oxysterols accumulate over time. E.g. egg yolk powder had ~110.5 µg/g 7-KC and similar 25-OH after storage; 1-year stored egg powder had 21–137 µg/g total oxysterols . (Lab analysis)
Scrambled eggs from egg powder (high intake per serving) Using oxidized powder can greatly increase COP intake. Human study: a test meal of scrambled eggs made with egg powder (plus butter/milk) provided ~11.5 mg of COPs – vastly higher than fresh eggs.

Sources: Analytical values from Refs ; human meal data from Ref .

Oxysterols in Dried and Processed Egg Products

As the table highlights, powdered egg products (spray-dried or powdered eggs) can contain significantly more oxysterols than cooked fresh eggs. When eggs are dried at high temperatures and exposed to air, some cholesterol oxidizes during processing and storage. Freshly made high-quality egg powder may start with low 7-KC (on the order of a few µg/g or less ), especially if drying is done under controlled conditions. However, upon storage, cholesterol oxidation continues: studies have found that spray-dried egg powder stored at room temperature can see COP levels double or more (e.g. from ~18 µg/g after drying to ~39 µg/g after some time in storage) . Commercial egg yolk powder in one analysis contained ~110 µg of 7-ketocholesterol per gram (and a similar level of 25-hydroxycholesterol) , reflecting substantial oxidation. Likewise, van de Bovenkamp et al. reported that fresh egg yolk had only ~1.2 µg/g total oxysterols, whereas whole egg powders stored 1 year contained 21–137 µg/g . These are chemical analysis results from food composition studies.

The practical implication is that dried egg products (e.g. powdered eggs used in baking mixes, long-shelf-life meals, etc.) contribute far more oxysterols than freshly cooked eggs. This was vividly demonstrated in a human dietary study by Emanuel et al. (1991), in which volunteers consumed scrambled eggs prepared from oxidized egg powder. That single meal delivered an estimated 11.5 mg of oxysterols (i.e. 11,500 µg of COPs) – orders of magnitude above what a fresh egg meal would provide. Subsequent plasma measurements showed significant absorption of those oxysterols . By contrast, a meal of fresh scrambled eggs would contain only a few hundred micrograms of COPs at most.

Conclusion

Fresh egg dishes like scrambled, fried, poached, or hard-boiled eggs generally have low levels of oxysterols. 7-Ketocholesterol in a fresh scrambled or fried egg is on the order of 0.1–0.3 mg per serving (a few µg per gram) , while boiled or poached eggs are even lower, often with negligible detectable 7-KC . These data come from direct chemical analyses of eggs under various cooking conditions. In contrast, industrial egg products (powdered eggs) can accumulate much higher oxysterol levels (dozens of µg/g), especially with prolonged storage . Regulatory and food safety reviews (e.g. EFSA) recognize eggs and egg products as a major source of dietary oxysterols , prompting interest in antioxidant strategies to limit cholesterol oxidation during processing. From a health standpoint, cooking eggs gently (boiling, poaching) minimizes oxysterol formation, whereas aggressive heating or using pre-oxidized egg powders can greatly increase oxysterol intake. Fortunately, for the average consumer eating fresh eggs, the absolute amounts of 7-ketocholesterol and related COPs are very smallscrambled eggs from fresh whole eggs contain only minor oxysterol levels relative to the total cholesterol present.

Sources:

  • Van de Bovenkamp et al. (1988) – Lipids 23(11):1079-85. [Chemical analysis of oxysterols in fresh vs stored egg products] .
  • Ren et al. (2013) – J. Agric. Food Chem. 61:11595-602. [Lab study on n-3 enriched eggs: COPs in fried vs boiled eggs] .
  • Cortinas et al. (2003) – J. Sci. Food Agric. 83:820-829. [Egg enrichment & processing; reported oxidation differences between boiled and scrambled (TBARS)] .
  • Rodriguez-Estrada et al. (2000) – Food Chem. 68: at 97-103. [Reported 7-KC in various foods; fried vs raw yolk, egg powder] .
  • Valenzuela et al. (2003) – Biol. Res. 36:291-302. [Review of dietary oxysterols; eggs as major source] .
  • Emanuel et al. (1991) – study cited in García-Llatas & Rodríguez-Estrada (2014) (Biochem. Biophys. Res. Commun. 446:792-7). [Human trial feeding scrambled egg powder meal, ~11.5 mg COP intake] .

o3-pro was wrong…