Starting up using a 2.0 ATA HBOT chamber, documenting the project step by step for publishing on biohacking project I am working on.

I want to find a reasonable:

  • ⁠o2 sensor (that can go more than 30%)
  • ⁠pressure sensor
  • co2 sensor

The reason is that i don’t want to rely on the software of the hbot chamber to measure the o2 level, by itself, being a critical safety measure.

The chamber that don’t uses a BIBS mask breathing system, which input o2 and output co2 in different tubes, are more comfortable, but carry an extra risks because more o2 leaks into the chamber.

My hbot chamber measure if o2 level reach 35% and in such case activate an industrial chiller that change the air inside the chamber and bring back the normal oxygen level of 21% .

I do want to measure independently the o2 value %, to be visible enabling the uses to manually enact safety procedures (reduce oxygen level input, activate industrial chiller, starts de-pressure procedure to exit) if o2 level goes beyond safety level %.

Did anyone had experience with that independent hbot in-chambers parameters measurement?

Fabio https://hackingbiology.com

Ps @RapAdmin would be nice to have a tag for hbot in the forum

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FWIW…

It is never a good idea to increase O² in a “chambers” atm fire risk, use bibs

Review

The diving industry standard equipment…

I technically agree with you on being best to have a BIBS, while it’s true that’s way more invasive to be dressed 4-5hours per week, also requiring swapping between different users for hygiene reasons.

I got that cheaper single-seat 2.0 HBOT chamber and now working around it, before uses, from all safety corners (for electricity waiting to receive an UPS with triple the electrical continuity time for decompression, plus a dedicated grounding checks).

While the uses is meant to be with mask, without a bibs there will be leaks into the chamber of both o2 and co2.

The EU EN 14931 and US NFPA 99 do require to stay within the limits of;

  • max 23.5% o2
  • max 0.5% co2

While the Chinese supplier say to respect those numbers and provides an industrial chiller for continuous air change:

and while there’s a fancy UI with some data including O2-level of the chamber itself:

I wouldn’t trust it and before putting myself into that chamber do want to have an independent measurements of o2/co2/ppm to verify the statements related to the air refreshments.

Just checking in… how is your HBOT chamber working out for you? Are you using it regularly yet? Any benefits you can quantify?

I believe you purchased this device, is that correct?

I have not yet started using it, mostly still very busy on closing business stuff to let me have a bright 2026 in biohacking.

What I have been doing up to now is:

  1. Received 2700watt UPS
  2. Tested electrical grounding
  3. Tested air quality inside using awair (that i use in the house, very high level of chemicals)
  4. Tested CO2 level inside
  5. Tested O2 environment level inside (several surprise, during compression time O2 % spike a lot and there’s disagreement between my O2 sensor and embedded O2 sensor that i require to spent a full day testing/documenting various scenarios)
  6. Just received, not yet tested, the oxygen precision tester the oxygen level from the oxygen concentrator (because the O2 is modulated, in order to balance the inside chamber air pressure, want to check it directly from the little tube)
  7. Did some deep diving with the manufacturer on the advanced technical settings, specifically related to the insider O2 sensor min-max threshold that should enact two security features 7.1 air refresh of the chamber (by my undertanding using the o2 compressor reducing injecting O2 but switching to fresh air) 7.2 stop O2 injection + starts decompression)
  8. Improve some electrical safety inside with self-meltdown electrical tape on 24v exposed rod

I do want to finalize my testing and in doing so write a report for the supplier but also a guideline for biohacker on how to select an HBOT chamber and how to test it, especially when buying it from a manufacturer far away in China.

In this moment i do not recommend, to buy from the supplier I brought the hbot chamber that’s https://tecnotu.com because of all the issues i am encountering by my own independent testing.

From my reading across forums, i found out that several of those issues are “common” but as @John_Hemming said, only goes with a chamber with a BIBS (and still, ensure independent testing).

I am engaging also with other suppliers, there’s some bigger chamber with BIBS also cheaper, obviously there’s always the issue of bringing it “inside the home” if it’s too big, can share some additional product offer i am receiving.

Btw my plan for uses, once I fixed and/or put under strict monitoring the issues, is to make uses 3 times a week of HBOT with 60min full sessions (so total 75min including ~15min for safe compression/decompression) as a standard practice.

While reading scientific papers there’s a lot of changes and improvements, that can be tracked down by blood work and microbiome, the KPI I do care is to extend telomere, actually measured at 7.2kbase with truage.

Fabio

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What was the cost of the chamber by itself?

The chamber with 20L oxygen concentrator and industrial chiller for temperature control, including shipping DDP (so also VAT&Duties) was $19.5k.

I found several other supplier with bigger, always 2.0 ATA pressure, also with BIBS and CO2 sensor for slightly less money.

But now I’d like to finalize this testing and make it working, as I’d also wish to come up in having software modification to modulate oxygen to do hyperoxia-normoxia automatically, without having to click every 15-5minutes.
At this point considering making an opensource hbot chamber partnering with one of those many suppliers :stuck_out_tongue_winking_eye:

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