#497

Yes, I do, but better to read it yourself. https://senolyticstreatment.com/

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#498

That would be my biggest concern, destroying the gut microbiome. That is what antibiotics are very good at.

But this protocol should be a cycled process, on for a short time and off for a longer time. Then with high dose pro and pre-biotics you could most likely rebuild the microbiome.

The Viome tests could show how that is going with before and after.

#499

As posed in September 2023 see link below.

#500

“The DAV protocol reduces senescence cells by over 90%.”

I’d like to know how that was tested?

There are many types of senescent cells and in many different parts of the body and there is NO single test that can tell anyone how many senescent cells are in any particular human.

Only through tissue biopsies can an actual senescent cell count be accomplished. And you would need biopsies in every tissue type to know, skin biopsies, lung biopsies, brain biopsies, etc.

There is no “general” marker that is reliable enough to make a statement like 90% clearance of senescent cells. Not even SA-ÎČ-Gal can be used as a general indicator, only when done on tissue biopsies is it even close to telling the tale of clearing senescent cells.

https://onlinelibrary.wiley.com/doi/full/10.1111/j.1474-9726.2006.00199.x

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#501

At proper dosing no anti microbal action.

#502

I’d be a bit skeptical on that and would definitely want to test my gut microbiome to be sure. For me personally, there are other ways to clear senescent cells with less risk.

I do lots of other “risky” things so I do get the risk reward thing :slight_smile:

#503

CD26 and metabolic flux{using a Seahorse Anlyzer

Read in detail the published paper, post at the start of this thread.

Review/watch the interview with Professor LIsanti, post in this thread, as the saying; “you will hear it from the horses mouth”.

And you could contact him, details is above posts and here.

Prof Michael Lisanti MD PhD

M.P.Lisanti@salford.ac.uk

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#504

Artigo completo:

https://sci-hub.wf/10.18632/aging.101905

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#505

After reading the paper, the study was done on cell cultures, not on humans.

The Seahorse XFe96 analyzer was used on cell cultures, not on living human derived biopsy samples. So my statement that clearing 90% of senescent cells in humans, remains unproven. I do remain hopeful that there will be positive results in humans :slight_smile:

Analyzing very specific cell cultures in vitro is the first step in developing protocols for humans. It’s a long way from a definitive treatment. When they get to in vivo testing, it will be very interesting to see the results.

While promising in vitro, human trials will tell the tale.

Measuring senescence in humans is a complex and expensive process.

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#506

The horse doesn’t say much about any human test results for senescence.

I do see some results in other studies, in humans, related to cancer for Doxycycline alone.

“Given these promising results in the ABC pilot study, here we aimed to further potentiate the efficacy of Doxycycline, for patient benefit. Our preliminary in vitro results indicate that the inhibitory effects of Doxycycline on CSC propagation can be further potentiated, by employing a combination therapy strategy, with two additional pharmacological agents, namely i) Azithromycin and ii) Vitamin C. Azithromycin inhibits the large mitochondrial ribosome, as an off-target side-effect. Vitamin C acts as a mild pro-oxidant, which can produce free radicals and, as a consequence, induces mitochondrial biogenesis.”

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#507

A small group of breast cancer “patients” where treat (with the DAV Therapy) at the University of Pisa by Federica Sotgia .

She is also his wife.

Do not take this the wrong way.

I have nothing to prove to anyone.

I do my own research and make my own decisions.

You can replicate any tests published in any paper.

All that is required are the Re$ource$

Contact

Prof Michael Lisanti MD PhD

M.P.Lisanti@salford.ac.uk

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#508

This is one of the first papers I read back in 2018 on Azithromycin as a senolytic. I got pretty excited about it back then as it’s very effective in vitro. So I get why people are interested in finding a way to get it to work in humans.

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#509

A Lisanti and Sotgia paper

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#510

The DAV protocol was not used in the ABC study on breast cancer.

From the study you have linked to it indicates a mono-therapy, Doxycycline only


“Antibiotic for Breast Cancer (ABC) trial was conducted at The University of Pisa Hospital [5]. The ABC trial aimed to assess the anti-proliferative and anti-CSC mechanistic actions of Doxycycline in early breast cancer patients [5]. The primary endpoint of the ABC trial was to determine whether short-term (2 weeks) pre-operative treatment with oral Doxycycline”

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#512

You would never cure any bacterial infection with a 50mg dose. The bacteria would be eating the Azh.

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#513

SNK,

Have you done the DAV Therapy/Protocol?

#514

From the Published Paper

DISCUSSION

In this report, we provide evidence supporting a novel “synthetic-metabolic” approach to target CSCs, via a triple combination therapeutic strategy, which includes two clinically-approved drugs and one essential vitamin. This therapeutic strategy drives the near complete elimination of CSC propagation, but only uses very minute amounts of these compounds. More specifically, this approach involves the simultaneous inhibition of two key targets, namely the large and small mitochondrial ribosomes. Because mitochondria originally evolved from bacteria over 1.45 billion years, they still share certain conserved features related to protein translation. As a consequence, Azithromycin specifically blocks the function of the large mito-ribosome (39S), as an off-target effect. Similarly, Doxycycline inhibits the small mito-ribosome (28S), also as an off-target effect. In contrast, Vitamin C functions as a mild pro-oxidant, producing free radicals. Here, we show that a triple combination of Doxycycline (1 ÎŒM), Azithromycin (1 ÎŒM) and Vitamin C (250 ÎŒM) effectively blocked CSC propagation by ~90% (summarized in Figure 14). In this context, we used the ER(+) breast cancer cell line, namely MCF7, as an established model system for monitoring and quantitating the 3D propagation of CSCs. Finally, we directly validated and confirmed that this DAV triple combination therapy potently inhibited mitochondrial oxygen consumption (OCR) and increased glycolytic flux (ECAR), as predicted. Based on our current metabolic findings, we propose that this triple combination is a feasible and novel anti-cancer strategy for targeting CSCs.

An external file that holds a picture, illustration, etc. Object name is aging-11-101905-g014.jpg

Figure 14

Summary diagram highlighting the mechanism(s) of action related to the triple combination of Azithromycin, Doxycycline and Vitamin C. This approach effectively results in the synergistic eradication of CSCs, using vanishingly small quantities of antibiotics. It is important to note Doxycycline and Azithromycin are not direct OXPHOS inhibitors, but instead are inhibitors of mitochondrial protein translation. The 2 metabolic targets are the large mito-ribosome and the small mito-ribosome. Azithromycin inhibits the large mitochondrial ribosome as an off-target side-effect. In addition, Doxycycline inhibits the small mitochondrial ribosome as an off-target side-effect. Vitamin C acts as a mild pro-oxidant and can stimulate the production of free radicals, driving mitochondrial biogenesis, secondary to mitochondrial oxidative stress and the anti-oxidant response. Vitamin C is also thought to act as an inhibitor of the glycolytic enzyme GAPDH (Glyceraldehyde 3-phosphate dehydrogenase). However, here, we did not observe any inhibition of glycolysis, when Vitamin C was tested alone.

As I have post many times;

*“If you wait until you are ready, it is almost certainly too late.”
~ Seth Godin

“I am not the waiting type.”
~Joseph

2 Likes
#515

Yes, I did first time around but for 5 days ONLY. I started yesterday and was all pumped up to do the whole 21 days, until @anuser crushed the party and scared the living daylights out of me and now I’ll do only three days and with doxy and Vit C only.

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#516

You should do your own research and make your own decisions.

Why do you pay any attention to a person who has absolutely no idea what the are speaking/posting about?

I do not waste my time, everything posted by him I block.

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#517

I think Steve finished it.

The DAV protocol wasn’t even used in at the University of Pisa Hospital, only doxycycline, as he mentioned.

The (lack) of evidence for benefit, and the evidence of harm speaks for itself. I do kind of enjoy the psychology of it, this thread is 101 why you should never, ever, attach any ‘protocol’ to your identity, just base your decisions on evidence, and risk-reward.

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