Ora Biomedical Million Molecule Challenge Results

[Curious]

So far I am at 800 $, one more person is interested but have not given a specific amount of $.

[Neo]

@Curious Ok, I can add $200

Can you ask the person you mentioned how much they can do and then perhaps when others see that we are close they can chip in a bit

[LGalindo]

I can contribute 100 dollars

[Curious]

@Curious

$1,583.72.

So far:
Curious - $400
@adssx - $400
@Neo - $200
@LGalindo - $100
@Josh are you interested?

[Josh]

Yah i just emailed them through lifespan to get an invoice without tax and see what they say. Hopefully i can cover the remainder.

[Josh]

Lifespan is forwarding request to ora to double check quote, but it sounds like it will work with payment mechanism etc

[relaxedmeatball]

If there’s some mechanism for payment sharing, I’m happy to chip in $100. (I’m not in the US, and don’t want to mess with international transfers etc.)

[Curious]

I have mailed them and asked them to let the experiment run till all the animals are dead. Having a cut of time at 30 days and then actively kill the animals (worms) that are still alive is not meaningful. In my experiment, 70 _ 75 % of the animals were still alive at day 30. And medium lifespan was not possible to calculate.

Having a cut-off date for an experiment is good when trying to screen lots of substances in a short time. But when you find substances that stand out, then there is a need to dig deeper, be more meticulous and find harder data. And that is where we are now.

[relaxedmeatball]

I totally agree, but it might be something Ora isn’t interested in, since throughput is obviously their aim.

I am wondering whether an academic lab which handles C.elegans might want to take on the testing of the most promising compounds? It seems like it wouldn’t be that difficult to set up, testing a few different doses.

[Mitchell_Ora]

Hello MMC sponsors!

Mitchell from Ora Biomedical here.

I just discovered this forum and it’s awesome! I’m so excited to see the community of MMC sponsors discussing results and how to push the boundaries of longevity science forward!

Thank you all again for participating in this first-of-its-kind citizen science driven effort to build the world’s largest open access longevity interventions database. You are pioneers on this journey with us and we could not do this without your support.

A quick update: we have a small mountain of longevity tests we are currently QC’ing and processing for release. I apologize again for the delays you have seen. We are a small team working with a tight budget. Until last November, all of this work was being done with just three people. We have grown our research team and have scaled our experimentation significantly. The next growing pain in this scaling is making our QC and data analysis pipeline as quick as possible. This is being solved as we speak.

Thank you all again and be on the lookout for your data! I am not sure how much time I will have to participate in the forum, but do not hesitate to reach out if you’d like to discuss how we can further advance longevity science together!

Best wishes all!

ml

[Curious]

I got a mail from Dr. Mitchell Lee. They are in full support of our crowdfunding efforts but thay dont have that feature built into their website yet.

“We can also keep the experiment running until all animals are dead. The doxycycline results are incredibly impressive, and we also want to see maximum lifespan produced, as well as if there are effects in the context of UV-killed food.”

[Josh]

I didnt get a reply and i wonder if they misunderstood my email through lifespan. I was thinking as long as most of the people who said theyd pitch it can reimburse me i think it will be fine. Lifespan may even be able to coordinate it as well if people use the custom donate and everyone did it within a couple days…

[adssx]

Carvedilol 50uM + Doxycycline HCl 50uM (based on this hypothesis: Ora Biomedical Million Molecule Challenge Results - #215 by adssx poke @Curious )

Nebivolol:

Cyclosporin A:

Verapamil:

Naringenin:

Magnolol:

Tauroursodeoxycholic Acid (TUDCA):

Weird result for TUDCA:

• UDCA did +5%: Ora Biomedical Million Molecule Challenge Results - #317 by adssx
• UDCA failed in the ITP.
• TUDCA is formed when taurine binds to UDCA. Then TUDCA metabolises to UDCA in the body. Some people consider TUDCA better than UDCA.
• A Chinese paper from 2024 found that TUDCA but not UDCA extended lifespan in worms: UDCA (ursodiol) / TUDCA for healthspan and lifespan? - #4 by adssx . The Chinese tested 2.5, 10, and 50 mmol/L TUDCA and found lifespan increases for all of them, the largest one with 10 mmol/L. They also tested UDCA 10 mmol/L and found no lifespan increase. Ora Biomedical tested 50 umol/L TUDCA and 10 umol/L UDCA. So, I’ll retest TUDCA and UDCA with a dose-response: 2.5, 10, and 50 mmol/L…

[John_Hemming]

One difficulty with Doxycyline is that it is quite good at killing the worm’s food (E Coli) before the food kills the worms.

[Curious]

If feeding the worms food that’s already killed, then we take that effect out of the equation? The effect that doxycycline can have confounding effects on the living food?

[Curious]

I see that in your now reported experiments, as well as in mine, the controls live longer than they did in some of the earlier experiments. There might also be a smaller variation in the length of the lifespans of the control groups.

[John_Hemming]

Thats true, but AFAICS these are experiments with live food.

[Yoo]

Caloric restriction?

[John_Hemming]

Escherichia coli (AFAIK)

[Josh]

It is both effects.
Ora said “With many of the antibiotic effects we have seen, the animals end up eating through the bacteria during the assay and likely become dietarily restricted in late life. That makes it very difficult to decouple between DR and suppression of dysbiosis (which is known to shorten worm lifespan) when assessing longevity effects.”