Neo
#21
Lol. Perhaps from that perspective. A lot of other reasons why it may be less so.
One of Stephanie Venn-Watson’s papers says "“Epidemiological, in vivo, and in vitro studies support the notion that 100 to 300 mg of C15:0 is needed daily to effectively achieve and maintain active circulating C15:0 concentrations of 10 to 30 µM [2].” The paper is “Pentadecanoic Acid (C15:0), an Essential Fatty Acid, Shares Clinically Relevant Cell-Based Activities with Leading Longevity-Enhancing Compounds,” Nutrients 2023, 15(21), 4607; Nutrients | Free Full-Text | Pentadecanoic Acid (C15:0), an Essential Fatty Acid, Shares Clinically Relevant Cell-Based Activities with Leading Longevity-Enhancing Compounds.
Some people like me don’t consume milk, cream, or beef. I do consume butter but I try to limit the quantity. So supplementation might make sense for some people.
I greatly limited dairy after I asked a urologist what to do about BPH. She told me to cut out dairy, eat more broccoli, and drink green tea or take EGCg. I did that and my PSA score went down for the first time ever, probably from the EGCg.
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JuanDaw
#23
You could have fish instead; unless you dislike them like desertshores.
Bluefin tuna (the fatty part, favored for sashimi), has 90 mg per 100 g. Might be one of the factors for the long life of the Japanese; tuna provides both C15 and omega 3.
The top scorer is a canned product.
Too bad sardines (nucleic acids) do not have high C15. So now I will fry my sardine omelette in butter, not EVOO.
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jnorm
#24
The plasma C15:0 concentrations for health benefits would likely be 5-20 µM, which is 10-40x lower than its IC50 in the HDAC6 inhibition assay.
Of course free intracellular C15:0 might be much lower than plasma concentrations, due to distribution, esterification, FABPs, etc.
a single oral dose of C15:0 at 35 mg/kg succeeded in achieving our targeted active plasma concentrations in this rodent model, between 2.5 to 5 µg/ml (equivalent to 6.7 to 20 µM), from 1 to 8 hours post-dose.
Efficacy of dietary odd-chain saturated fatty acid pentadecanoic acid parallels broad associated health benefits in humans: could it be essential?
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JuanDaw
#25
@ JamesPaul108]
Thank you. Just read your link now. Seems C15 provides benefits provided by rapamycin.
C15:0 and rapamycin have the most dose-dependent and clinically relevant activities. Full cell-based phenotypic BioMAP Diversity PLUS profiles for each of the four compounds are provided in the Supplementary Materials (Table S1). Of the four compounds tested, pure C15:0 (FA15) had the most dose-dependent annotated activities (i.e., had 2 or more consecutive concentrations changed in the same direction relative to vehicle controls, were outside the significance envelope, and had at least one concentration with an effect size >20% (|log10 ratio| > 0.1)) (n = 36), closely followed by rapamycin (n = 32) (Table 1).
C15:0 (1.9–50 µM) shared 12 annotated, dose-dependent cell-based activities with rapamycin (0.3–9 µM) across 7 (58%) out of 12 cell systems (Table 2).
At their optimal doses, C15:0 (17 µM) shared 24 significant cell-based activities with rapamycin (9 µM) across 10 (83%) out of 12 cell systems (Table 4).
Our findings are consistent with previously demonstrated anti-inflammatory, antifibrotic, anticancer, and antimicrobial effects of C15:0 and rapamycin in vitro and in vivo [2,26,27,28,57,58,59]. These common activities between C15:0 and rapamycin, which are reasonably expected to enhance healthspan, may be due in part to a shared mTOR-inhibiting mechanism that is a key target for enhancing longevity [23,56].
Consistent with metformin as a treatment for type 2 diabetes, pure C15:0 has been shown to improve glucose uptake by cells, and supplementation over 12 weeks effectively lowers glucose, cholesterol, MCP-1, and IL-6 in a high-fat diet-induced mouse model of type 2 diabetes [2,22]. Further, numerous prospective cohort studies have consistently shown that people with higher circulating C15:0 concentrations have a lower risk of having or developing type 2 diabetes, gestational diabetes, and diabetic retinopathy [3,4,5,6,76,77]. As such, there is a need for clinical trials to evaluate C15:0 as an essential fatty acid that, similar to metformin, may help prevent and attenuate type 2 diabetes.
Engadin
#27
Among both Nature and Researchgate studies authors is some Stephanie Venn-Watson who helped with study protocol designs, study oversight, data analysis, data interpretation and manuscript writing in Nature’s study. And in Researchgate’s she is deeply involved in the study in the following areas:
Conceptualization: Stephanie K. Venn-Watson.
Investigation: Stephanie K. Venn-Watson.
Project administration: Stephanie K. Venn-Watson, Camden N. Butterworth.
Resources: Stephanie K. Venn-Watson.
Supervision: Stephanie K. Venn-Watson.
Validation: Stephanie K. Venn-Watson.
Writing – original draft: Stephanie K. Venn-Watson, Camden N. Butterworth.
Writing – review & editing: Stephanie K. Venn-Watson, Camden N. Butterworth.
Stephanie K. Venn-Watson affiliations:
- Seraphina Therapeutics, Inc., San Diego, CA, 92106, USAStephanie Venn-Watson
- Epitracker Inc., San Diego, CA, 92106, USAStephanie Venn-Watson & Richard Lumpkin
Both Seraphina Therapeutics and Epitracker Inc. are C15:00 producers and marketeers.
I would take both studies results, if not all with this person involved, with a tiny, tiny pinch of salt. To say the least, of course.
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KarlT
#28
Does anyone know why C15 is classified as “essential”? Why does it do that is a requirement in humans?
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The video said our bodies make some but not enough so we need to get a small amount from the diet (or supplementation). I’m not clear on how they figured out how much we “need”.
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José
#30
C15 coffee
Fresh ground and brewed dark coffee
Add 12g of glycine, then 2 tbsp of salted butter with 82% butter fat.
Optional splash of heavy cream
Your daily dose of “natural” C15
2 tbsp of butter = 264mg
2 tbsp of salted butter = 239mg
cream per tbsp = 67.5mg
- Commodity butter has about 80% butterfat
- European butter has beetween 82-83% butterfat
- Minerva Dairy butter has the highest fat content with 85% butterfat
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José
#31
fatty15 - 3g/3,000mg (100mg x 30 capsules) $50.00
10g of Pentadecanoic acid, 99%,
$70.00
50g of Pentadecanoic acid, 99%,
$175.00
At 300mg a day you would used 9g per month
3g ever 10 days
Bicep
#32
Where did you see these prices?
José
#34
The fatty15 supplement has been post.
The Pentadecanoic acid, 99%, a standard chemical supplier.
“fatty15” is manufactured for Seraphina Therapeutics.
José
#35
FWIW
Review the patent, owned by US Navy{US tax dollars paid for this] This patent is licensed to Seraphina Therapeutics.
Patent has dosing details by mg per lb
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I think the source of this Pentadecanoic acid ¶ is Thermo Scientific Chemicals who are owned by Thermo Fisher Scientific Inc. “an American supplier of analytical instruments, life sciences solutions, specialty diagnostics, laboratory, pharmaceutical and biotechnology services”. They acquired Alfa Aesar in 2015 and are based in Waltham, Massachusetts, Thermo Fisher was formed through the merger of Thermo Electron and Fisher Scientific in 2006. I think Alfa Aesar were originally Based in Heysham, U.K?
Pentadecanoic acid is a lipid used in proteomics research. It is also used as a biomarker for dietary milk intake. It can be labelled as a biomarker for myocardial fatty acid metabolism in ischemia/reperfusion studies.
This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.
The product is available to companies only. The production specification datasheet is dated 3/17/2024 so it appears to be current.
I haven’t tracked down the link back to the US Navy, if one exits.
I found an old Amazon product page posted by Alfa Aesar selling PA but it’s not current or in stock.
That’s all the sleuthing I have time for tonight but it looks like a real potential source of Pentadecanoic acid at a significantly reduced cost.
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Under patent law a naturally occurring substance, that hasn’t been modified, can’t be patented. This could be similar to the Chroma Dex NR story?
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@AlexKChen
“…it claims that the body needs 200-300 mg of C15 (at 06:18 in the video) to meet our minimum daily requirements…”
Did you come away with an understanding of how they came to the conclusion that we needed an exogenous supply of 200-300 mcg of C15? An “essential” fatty acid, as I understand it.
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Thanks to all above for running down these many issues. A great example of the benefits of this forum.
A random thought. There is a slight chance that those of us taking ezetimibe are robbing ourselves of whatever C15 we consume in our diet, including via supplement. My read of ezetimibe’s highly selective acton on NPC1L1 protein suggests it would not interfere with the adsorption of C15:0 but these matters are complex, including binding, and not fully understood. In general, I maintain concerns about what important fats ezetimibe is reducing the assimilation of.
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