AnUser
#1
Hello all,
I know about the Aging 3.0 AI and Levine age calculators and the blood biomarkers that they are based on. Unfortunately I cannot get one or two blood tests required for each test, like RBW or total protein.
I am interested in what you think are the best biomarkers to track for improving healthspan and longevity. I will use it to measure the effects of any supplements or medications I take and I am sure it will be useful to others.
I am specifically interested in the most important biomarkers to start with.
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JKPrime
#2
Something like this
combined with AI assessment of pics for your perceived age. Further, Iām not convinced that any of the aging clocks are of much value here.
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AnUser
#3
Interesting, so you donāt believe that measuring blood biomarkers are that important?
CRP gives an indication of senescence load, but you need to measure it a few times to exclude infection.
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AnUser
#5
Is that your most important biomarker? Do you have any others that you think are important?
I think that is the most important. However, the usual ones about organ function are relevant as well. I prefer Cystatin-C to Creatinine. I would prefer wearing a CGM to monitor glucose to HbA1c, but HbA1c is useful. In terms of cardiac function Blood Pressure, RHR and HRV are relevant as well as things like Creatinine Kinase. I would not want to give a definitive list. Generally the more the merrier.
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Ulf
#7
ApoB, vo2max, fasting glucose, high-sensitive CRP and lymphocytes or neutrophil/ lymphocyte ratio are those I rate the highest.
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Itās a great question. I think the fundamentals are the most important. Iām trying to sort out what to track, such as: apoB (ASCVD), HbA1c & HOMA-IR & body fat% (metabolic health), blood pressure and RHR (cardiovascular & mitochondrial health), hsCRP (immune system), Cystatin-C (kidney), HRV (adequate sleep & stress mgmt).
What am I missing?
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AnUser
#9
My HRV is extremely low, so thatās something Iām looking to increase. Itās around 27 ms which I believe is below the 5th percentile. In fact it was 32 ms in 2021, 29 ms in 2022 and 27 ms this year (measured daily). I have never exercised regularly which might be the reason. I am kind of deliberately deconditioned because I want to see how well I will improve.
Do you have an optimal range for those blood markers and how do you determine it?
Why do you think lymphocytes or the neutrophil/lymphycyte ratio is important to measure, is it because youāre taking rapamycin? Or is it important for everyone else too?
Yes, I am measuring apoB already. Itās top of my list, I donāt know about anything else right now.
Why is Cystatin-C better than Creatinine?
How do you measure HOMA-IR and why is it important?
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scta123
#10
Those are really standard lab bloodwork test. Any lab can do it.
RBW is part of blood differential test
total protein is also a standard test, but can be calculated from albumin, globulin and fibrinogen
If you are doing tests and will track them, I recommend that you track also kidney and liver biomarkers, lipids, glucose and insulin, minerals, vitamins, hormonesā¦
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scta123
#11
btw PA recommends his patients to track these:
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Creatinine can be misleading. Serum creatinine is a breakdown product of muscle. People with more muscle mass make more creatinine per day. Serum creatinine based equations underestimate GFR in patients with higher muscle mass.
HOMA-IR is a calculation based on fasting glucose and fasting insulin. Itās a marker of insulin resistanceā¦.metabolic flexibility.
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apoB (ASCVD), HbA1c & HOMA-IR & body fat% (metabolic health), blood pressure and RHR (cardiovascular & mitochondrial health), hsCRP (immune system),
I think these are the important ones, everything else is (perhaps) nice to have but not gotta have.
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cl-user
#15
Fasting glucose is totally meaningless.
I have a GCM and here is a typical fasted glucose plot from midnight to noon.
My glucose goes down to 84 at 2:30 am then start to ramp up linearly from 2:30am to 9:15am
Then I go for a 10k run and the glucose first goes down to 80 before the liver decides to make some and it goes up to 114 at 10:44am when I stop running. During the cooldown the glucose goes down to 96 at noon.
All that has been fasted and the last meal was at 6pm the day before so, depending when you take that fasting glucose, it can be between anywhere between 80 and 114 which makes it a totally useless measurement. A 42.5% variation which will also translate into a 42.5% variation of HOMA-IR BTW.
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JKPrime
#16
Right. Unless of course you have an ongoing medical condition that is being actively treated, I wouldnāt obsess over the blood bio-markers. I would focus instead on sleeping, keeping health bmi and daily exercise.
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HRV asleep is different to awake. How do.you measure it?
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AnUser
#18
The response I got regarding RBW is that Entrocytes, Hemoglobin, EVF, MCV and MCH is used to get the same answer hence it is not used. Is it possible to do your own calculation with albumin, globulin and fibrinogen test?
Do you track them to see the general function of the kidney and liver or do you also check if theyāre optimal with regards to ACM from studies?
He doesnāt check entrocytes? Immune system?
It is average HRV throughout the night with an Oura ring.
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Jonas
#19
what would be the ideal neutrophil/lymphocyte ratio? Is age-adjusted?
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scta123
#20
Red cell distribution width (RDW) is an important biomarker and I doubt they would not do it. Just ask for differential blood count or blood differential test, sometimes it is called blood morphology test, it is always part of that.
Yes if you take the three protein test and add the values you get total protein.
I mostly track to check the function, sometimes I would check ACM too, in case of liver and kidney it mostly corresponds with optimal values, but many blood markers change trough life and what is considered optimal in young age might not be optimal in old ageā¦ and things get too complex for my preference for less structured approach. If you start looking at all fine details one might get lost. For example my RDW went up from 11,8 to 13,2% on rapamycin. It is still in normal range. All other biomarkers āimprovedā but this one is showing a trend that I donāt like. But should I now concentrate on this biomarker? Or just say, the average is better?
IDK what do you mean, but since he is tracking hemoglobin which is part of complete blood count probably you track others too. Immunity is tracked trough hs-CRP I guess.
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You will find that does not compare to people who measure it whilst awake (the latter will be higher). I use a Polar H10 combined with the elite software to measure HRV. I also have a fitbit HRV whilst asleep. The awake value is perhaps 20-30 milliseconds larger than the sleeping value. They vary at times in different directions.
IMO hrv varies too much on a daily basis to be an easy biomarker to get useful meaning from. Today my waking HRV was 60ms and sleeping (fitbit) was 41ms. Yesterday was 60/28, the day before 58/36.
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